MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function

Kang,Wei; Tong,Joanna H.M.; Chan,Anthony W.H.; Cheng,Alfred S.L.; Yu,Jun; To,Kafai

doi:10.3892/or.2014.3094

MCM7 serves as a prognostic marker in diffuse-type gastric adenocarcinoma and siRNA-mediated knockdown suppresses its oncogenic function

Authors:
- Wei Kang
- Joanna H.M. Tong
- Anthony W.H. Chan
- Alfred S.L. Cheng
- Jun Yu
- Kafai To
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Affiliations: Department of Anatomical and Cellular Pathology, State Key Laboratory in Oncology in South China, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China, School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China, Institute of Digestive Disease, Partner State Key Laboratory of Digestive Disease, Sir Y.K. Pao Cancer Center, The Chinese University of Hong Kong, Hong Kong, SAR, P.R. China
Published online on: March 19, 2014 https://doi.org/10.3892/or.2014.3094
Pages: 2071-2078

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Abstract

MCM7 (mini-chromosome maintenance protein 7) is essential for the initiation of genomic replication as a component of the pre-replication complex. The present study aimed to analyze its expression, clinical significance and biological functions in gastric adenocarcinoma (GAC). The MCM7 protein was upregulated in all 9 GAC cell lines. In 6 paired primary GACs, MCM7 was upregulated in tumor compared with the corresponding non-tumorous gastric tissues. In normal gastric epithelium tissue, MCM7 was strictly expressed in the proliferative compartment. MCM7 knockdown by siRNA in gastric cancer cell line AGS and NCI-N87 significantly suppressed cell proliferation, inhibited monolayer colony formation, reduced cell invasion and induced late apoptosis. Its nuclear expression correlated with advanced age and poorer disease specific survival in diffuse-type GACs. All the findings supported that MCM7 might play an oncogenic role in gastric tumorigenesis. It serves as a potential prognostic marker and therapeutic target in diffuse-type GACs.

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