Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2

  • Authors:
    • Li-Li Sun
    • Jian Wang
    • Zhi-Juan Zhao
    • Ning Liu
    • Ai-Lian Wang
    • Hua-Yan Ren
    • Fan Yang
    • Ke-Xin Diao
    • Wei-Neng Fu
    • En-Hua Wan
    • Xiao-Yi Mi
  • View Affiliations

  • Published online on: March 21, 2014     https://doi.org/10.3892/or.2014.3105
  • Pages: 2085-2092
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Abstract

TRAF2 promotes cancer cell survival, proliferation and metastasis through the NF-κB pathway by directly interacting with various TNF recepors. However, the molecular mechanism of TRAF2 dysregulation in breast cancer remains to be elucidated. In the present study, miR-502-5p was predicted as a potential regulator of TRAF2. miR-502-5p was significantly downregulated in breast cancer tissues when compared to the level in paired normal breast tissues. The breast cancer cell lines including MCF-7 and MDA-MB-231 expressed a lower level of miR-502-5p when compared to the level in the non-malignant breast epithelial cell line MCF-10A. In vitro, miR-502-5p enhanced early apoptosis and inhibited proliferation of breast cancer cells. Luciferase reporter assay results showed that miR-502-5p could bind to the 3'-untranslated region of the TRAF2 gene, thus, exerting an inhibitory effect on TRAF2. Furthermore, silencing of TRAF2 exhibited effects similar to those of exogenous miR‑502-5p, while overexpression of TRAF2 partially abrogated miR-502-5p-mediated suppression in breast cancer cells. In conclusion, miR-502-5p may act as a tumor-suppressor gene by targeting oncogenic TRAF2 in breast cancer and, therefore, may be a potential diagnostic and anticancer therapeutic marker for breast cancer.
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May-2014
Volume 31 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Sun L, Wang J, Zhao Z, Liu N, Wang A, Ren H, Yang F, Diao K, Fu W, Wan E, Wan E, et al: Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2. Oncol Rep 31: 2085-2092, 2014.
APA
Sun, L., Wang, J., Zhao, Z., Liu, N., Wang, A., Ren, H. ... Mi, X. (2014). Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2. Oncology Reports, 31, 2085-2092. https://doi.org/10.3892/or.2014.3105
MLA
Sun, L., Wang, J., Zhao, Z., Liu, N., Wang, A., Ren, H., Yang, F., Diao, K., Fu, W., Wan, E., Mi, X."Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2". Oncology Reports 31.5 (2014): 2085-2092.
Chicago
Sun, L., Wang, J., Zhao, Z., Liu, N., Wang, A., Ren, H., Yang, F., Diao, K., Fu, W., Wan, E., Mi, X."Suppressive role of miR-502-5p in breast cancer via downregulation of TRAF2". Oncology Reports 31, no. 5 (2014): 2085-2092. https://doi.org/10.3892/or.2014.3105