Caveolin-1 plays a key role in the oleanolic acid-induced apoptosis of HL-60 cells

Ma,Wei; Wang,Di-Di; Li,Li; Feng,Yu-Kuan; Gu,Hong-Mei; Zhu,Gui-Ming; Piao,Jin-Hua; Yang,Yu; Gao,Xu; Zhang,Peng-Xia

doi:10.3892/or.2014.3177

Caveolin-1 plays a key role in the oleanolic acid-induced apoptosis of HL-60 cells

Authors:
- Wei Ma
- Di-Di Wang
- Li Li
- Yu-Kuan Feng
- Hong-Mei Gu
- Gui-Ming Zhu
- Jin-Hua Piao
- Yu Yang
- Xu Gao
- Peng-Xia Zhang
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Affiliations: School of Basic Medicine, Jiamusi University, Jiamusi 154000, P.R. China, Department of Biology, Mudanjiang Medical University, Mudanjiang 157011, P.R. China, Department of Biochemistry, Harbin Medical University, Harbin 150086, P.R. China
Published online on: May 15, 2014 https://doi.org/10.3892/or.2014.3177
Pages: 293-301

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Abstract

Our previous study found that caveolin-1 (CAV-1) protein expression is upregulated during oleanolic acid (OA)-induced inhibition of proliferation and promotion of apoptosis in HL-60 cells. CAV-1 is the main structural protein component of caveolae, playing important roles in tumorigenesis and tumor development. It has been shown that cav-1 expression is lower in leukemia cancer cell lines SUP-B15, HL-60, THP-1 and K562 and in chronic lymphocytic leukemia primary (CLP) cells when compared with normal white blood cells, with the lowest cav-1 expression level found in HL-60 cells. To study the effects of cav-1 in HL-60 cells and the effects of cav-1 overexpression on OA drug efficacy, cav-1 was overexpressed in HL-60 cells using lentiviral-mediated transfection combined with OA treatment. The results showed that cav-1 overexpression inhibited HL-60 cell proliferation, promoted apoptosis, arrested the cell cycle in the G1 phase and inhibited activation of the PI3K/AKT/mTOR signaling pathway. Overexpression of CAV-1 also increased HL-60 cell sensitivity to OA. To further verify whether OA affects HL-60 cells via the activation of downstream signaling pathways by CAV-1, cav-1 gene expression was silenced using RNAi, and the cells were treated with OA to examine its efficacy. The results showed that after cav-1 silencing, OA had little effect on cell activity, apoptosis, the cell cycle and phosphorylation of HL-60 cells. This study is the first to show that CAV-1 plays a crucial role in the effects of OA on HL-60 cells.

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