Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model

Chien,Yi-Chun; Chen,John  Chun-Hao; Lin,Wei-Chan; Ding,Hueisch-Jy; Wang,Hsin-Ell; Kao,Chih-Hao  K.; Hwang,Jeng-Jong

doi:10.3892/or.2014.3256

Using [18F]FBAU for imaging brain tumor progression in an F98/tk-luc glioma-bearing rat model

Authors:
- Yi-Chun Chien
- John Chun-Hao Chen
- Wei-Chan Lin
- Hueisch-Jy Ding
- Hsin-Ell Wang
- Chih-Hao K. Kao
- Jeng-Jong Hwang
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Affiliations: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Bei-tou 11221, Taipei, Taiwan, R.O.C., Department of Radiology, Cathay General Hospital, Taipei 10630, Taiwan, R.O.C., Department of Medical Imaging and Radiological Sciences, I-Shou University, Jiaosu Village, Kaohsiung 82445, Taiwan, R.O.C., Department of Radiopharmaceutical Production, Buddhist Tzu Chi General Hospital, Hualien 97002, Taiwan, R.O.C.
Published online on: June 12, 2014 https://doi.org/10.3892/or.2014.3256
Pages: 691-699

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Abstract

1-(2-Deoxy-2-[18F]fluoro-β-D-arabinofuranosyl)-5-bromouracil ([18F]FBAU), a substitute for thymine, has been reported as an effective reporter probe by which to trace cellular metabolism with its positron emission. In the present study, a rat xenograft model bearing F98 glioma transfected with dual reporter genes, herpes simplex virus type 1 thymidine kinase (HSV1-tk) and firefly luciferase (luc) was used for monitoring tumor progression by multimodalities of molecular imaging using [18F]FBAU and D-luciferase as probes. Rat F98 glioma cells were transfected with the pC1-tk-IRES-luc vectors. The selected stable clone was renamed as the F98/tk-luc cell line. Fischer 344 male rats bearing orthotropic F98/tk-luc gliomas in the left brain were used. On day 13 post tumor inoculation, biodistribution, positron emission tomography (PET), magnetic resonance imaging (MRI) and ex vivo autoradiography were performed. The surviving fraction of F98/tk-luc cells treated with 15 µM ganciclovir (GCV) was 15.9%, and the uptake of [131I]FIAU in these cells was significantly enhanced when compared with F98 cells. The correlation coefficient of tumor volume vs. the bioluminescence in the F98/tk-luc glioma-bearing rats was 0.90. The biodistribution showed that the accumulation ratios of [18F]FBAU for glioma-to-normal brain were 9.16, 14.24, 5.7 and 13.7 at 30, 60, 90 and 120 min post i.v. injection, respectively. Consistent tumor enhancement of [18F]FBAU/PET imaging was also noted from 30-90 min post injection. Ex vivo autoradiography also confirmed significant [18F]FBAU uptake in tumors. In conclusion, [18F]FBAU may be used as a PET probe for monitoring glioma progression in animal models and may have potential for clinical use as well.

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