Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7‑TIPRL in human hepatocellular carcinoma cells

  • Authors:
    • Hyo-Jung Lee
    • Hyun-Soo Cho
    • Soo  Young Jun
    • Jeong-Ju  Lee
    • Ji‑Yong  Yoon
    • Jae-Hye  Lee
    • Hyuk‑Hwan Song
    • Sang  Ho Choi
    • Soo‑Yong  Kim
    • Vassiliki Saloura
    • Choon  Gil Park
    • Nam-Soon Kim
  • View Affiliations

  • Published online on: June 23, 2014     https://doi.org/10.3892/or.2014.3279
  • Pages: 1117-1123
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Abstract

Induction of apoptosis through activation of the TRAIL pathway is considered to be a promising anticancer strategy due to its ability to selectively induce apoptosis in cancer cells. However, the ability of cancer cells to acquire TRAIL resistance has limited the clinical translation of this approach. We previously reported that the TOR signaling pathway regulator-like (TIPRL) protein contributes to the resistance to TRAIL-induced apoptosis by inhibiting the MKK7-c-Jun N-terminal kinase (JNK) pathway via MKK7‑TIPRL interaction. In the present study, we identified Tussilago farfara L. (TF) as a novel TRAIL sensitizer among 500 natural products using an ELISA system that specifically detects the MKK7-TIPRL interaction, and we validated candidates by GST-pull down assay. Co-treatment of Huh7 cells with TF and TRAIL induced apoptosis via inhibition of the MKK7-TIPRL interaction and an increase in MKK7/JNK phosphorylation. This is the first report to describe TF as a novel TRAIL sensitizer, unveiling a potentially novel therapeutic strategy in cancer therapy.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee H, Cho H, Jun SY, Lee J, Yoon JY, Lee J, Song HH, Choi SH, Kim SY, Saloura V, Saloura V, et al: Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7‑TIPRL in human hepatocellular carcinoma cells. Oncol Rep 32: 1117-1123, 2014.
APA
Lee, H., Cho, H., Jun, S.Y., Lee, J., Yoon, J., Lee, J. ... Kim, N. (2014). Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7‑TIPRL in human hepatocellular carcinoma cells. Oncology Reports, 32, 1117-1123. https://doi.org/10.3892/or.2014.3279
MLA
Lee, H., Cho, H., Jun, S. Y., Lee, J., Yoon, J., Lee, J., Song, H., Choi, S. H., Kim, S., Saloura, V., Park, C. G., Kim, N."Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7‑TIPRL in human hepatocellular carcinoma cells". Oncology Reports 32.3 (2014): 1117-1123.
Chicago
Lee, H., Cho, H., Jun, S. Y., Lee, J., Yoon, J., Lee, J., Song, H., Choi, S. H., Kim, S., Saloura, V., Park, C. G., Kim, N."Tussilago farfara L. augments TRAIL-induced apoptosis through MKK7/JNK activation by inhibition of MKK7‑TIPRL in human hepatocellular carcinoma cells". Oncology Reports 32, no. 3 (2014): 1117-1123. https://doi.org/10.3892/or.2014.3279