Altered expression of ETV1 and its contribution to tumorigenic phenotypes in gastrointestinal stromal tumors

  • Authors:
    • Yan Zhang
    • Meng-Li Gu
    • Xin-Xin  Zhou
    • Han  Ma
    • Hang-Ping  Yao
    • Feng Ji
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  • Published online on: June 23, 2014     https://doi.org/10.3892/or.2014.3281
  • Pages: 927-934
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Abstract

Gastrointestinal stromal tumor (GIST) is the most common mesenchymal neoplasm of the gastrointestinal tract. ETV1 is a unique transcription factor specific to GIST that has been reported to date. The present study aimed to determine aberrant ETV1 expression and its contribution to tumorigenesis in GISTs. Altered expression levels of ETV1 and its relevant signaling pathways were assessed using western blotting and quantitative real-time PCR in 72 paired patient tissue samples. In addition, immunochemistry was performed on 156 GISTs using tissue microarray to analyze the correlation between ETV1 and clinical parameters. The results revealed that ETV1 was highly expressed in the GISTs at both the transcription and protein levels. Immunochemical analysis revealed that increased expression of ETV1 was correlated with KIT in the 156 patients. In addition, the frequency of ETV1 positivity was higher when compared with KIT [50.0% (9/18) vs 38.9% (7/18)] particularly in high-risk GISTs. Analysis of western blotting data showed that total protein isoforms of Raf, MEK and ERK were similar in the GIST tissues as well as in the uninvolved normal tissues. In contrast, the level of phospho-Raf, phospho-MEK and phospho‑ERK were decreased in the tumor group. Moreover, enhanced signaling molecules such as Bcl-2 and Dvl2/GSK-3β/β-catenin/Smad2 were detected, showing a significant difference in comparison with the uninvolved normal cases. We conclude that ETV1, a member of the ETS family, is upregulated in GISTs, and its signaling is integrated into a cellular signaling network for resistance to apoptosis, tumor cell invasion and survival.
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September-2014
Volume 32 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhang Y, Gu M, Zhou X, Ma H, Yao H and Ji F: Altered expression of ETV1 and its contribution to tumorigenic phenotypes in gastrointestinal stromal tumors. Oncol Rep 32: 927-934, 2014.
APA
Zhang, Y., Gu, M., Zhou, X., Ma, H., Yao, H., & Ji, F. (2014). Altered expression of ETV1 and its contribution to tumorigenic phenotypes in gastrointestinal stromal tumors. Oncology Reports, 32, 927-934. https://doi.org/10.3892/or.2014.3281
MLA
Zhang, Y., Gu, M., Zhou, X., Ma, H., Yao, H., Ji, F."Altered expression of ETV1 and its contribution to tumorigenic phenotypes in gastrointestinal stromal tumors". Oncology Reports 32.3 (2014): 927-934.
Chicago
Zhang, Y., Gu, M., Zhou, X., Ma, H., Yao, H., Ji, F."Altered expression of ETV1 and its contribution to tumorigenic phenotypes in gastrointestinal stromal tumors". Oncology Reports 32, no. 3 (2014): 927-934. https://doi.org/10.3892/or.2014.3281