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The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway

  • Authors:
    • Zhongliang Ma
    • Lin Hou
    • Yuhong Jiang
    • Yanpin Chen
    • Jinlian Song

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  • Published online on: August 20, 2014     https://doi.org/10.3892/or.2014.3426
  • Pages: 2111-2117
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Abstract

Isatin is an endogenous indole in mammalian tissues and fluids that is expected to have antitumor effects in human breast cancer cells. Human breast cancer cells (MCF-7) were exposed to isatin at various concentrations (0, 50, 100, 200 µmol/l) for 48 h. Apoptotic features were demonstrated by nuclei staining with Hoechst 33258 and flow cytometry. Bcl-2 and Bax mRNA were analyzed via reverse transcription-polymerase chain reaction. Bcl-2, Bax, the inhibitor of caspase-activated DNase (ICAD), and cytochrome c protein were analyzed by western blot analysis. Apoptosis, caspase-9 and -3 activation and mitochondrial depolarization were assayed by flow cytometry. The results showed that isatin induced apoptosis of MCF-7 cells. Furthermore, Bcl-2 expression was decreased and the ratio of Bcl-2 to Bax was significantly decreased by isatin. The mitochondrial transmembrane potential was markedly decreased and the release of cytochrome c into the cytosol was elevated following treatment with isatin. At the same time, caspase-9 and -3 were stimulated, followed by the degradation of ICAD, a caspase-3 substrate.
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November-2014
Volume 32 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Impact Factor: 3.8
  • Ranked Oncology
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  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Ma Z, Hou L, Jiang Y, Chen Y and Song J: The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncol Rep 32: 2111-2117, 2014.
APA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., & Song, J. (2014). The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway. Oncology Reports, 32, 2111-2117. https://doi.org/10.3892/or.2014.3426
MLA
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32.5 (2014): 2111-2117.
Chicago
Ma, Z., Hou, L., Jiang, Y., Chen, Y., Song, J."The endogenous oxindole isatin induces apoptosis of MCF‑7 breast cancer cells through a mitochondrial pathway". Oncology Reports 32, no. 5 (2014): 2111-2117. https://doi.org/10.3892/or.2014.3426