A novel RET rearrangement (ACBD5/RET) by pericentric inversion, inv(10)(p12.1;q11.2), in papillary thyroid cancer from an atomic bomb survivor exposed to high-dose radiation

  • Authors:
    • Kiyohiro Hamatani
    • Hidetaka Eguchi
    • Kazuaki  Koyama
    • Mayumi  Mukai
    • Kei Nakachi
    • Yoichiro Kusunoki
  • View Affiliations

  • Published online on: August 29, 2014     https://doi.org/10.3892/or.2014.3449
  • Pages: 1809-1814
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Abstract

During analysis of RET/PTC rearrangements in papillary thyroid cancer (PTC) among atomic bomb survivors, a cDNA fragment of a novel type of RET rearrangement was identified in a PTC patient exposed to a high radiation dose using the improved 5' RACE method. This gene resulted from the fusion of the 3' portion of RET containing tyrosine kinase domain to the 5' portion of the acyl-coenzyme A binding domain containing 5 (ACBD5) gene, by pericentric inversion inv(10)(p12.1;q11.2); expression of the fusion gene was confirmed by RT-PCR. ACBD5 gene is ubiquitously expressed in various human normal tissues including thyroid. Full-length cDNA of the ACBD5-RET gene was constructed and then examined for tumorigenicity. Enhanced phosphorylation of ERK proteins in the MAPK pathway was observed in NIH3T3 cells transfected with expression vector encoding the full-length ACBD5/RET cDNA, while this was not observed in the cells transfected with empty expression vector. Stable NIH3T3 transfectants with ACBD5-RET cDNA induced tumor formation after their injection into nude mice. These findings suggest that the ACBD5-RET rearrangement is causatively involved in the development of PTC.
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November-2014
Volume 32 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hamatani K, Eguchi H, Koyama K, Mukai M, Nakachi K and Kusunoki Y: A novel RET rearrangement (ACBD5/RET) by pericentric inversion, inv(10)(p12.1;q11.2), in papillary thyroid cancer from an atomic bomb survivor exposed to high-dose radiation. Oncol Rep 32: 1809-1814, 2014.
APA
Hamatani, K., Eguchi, H., Koyama, K., Mukai, M., Nakachi, K., & Kusunoki, Y. (2014). A novel RET rearrangement (ACBD5/RET) by pericentric inversion, inv(10)(p12.1;q11.2), in papillary thyroid cancer from an atomic bomb survivor exposed to high-dose radiation. Oncology Reports, 32, 1809-1814. https://doi.org/10.3892/or.2014.3449
MLA
Hamatani, K., Eguchi, H., Koyama, K., Mukai, M., Nakachi, K., Kusunoki, Y."A novel RET rearrangement (ACBD5/RET) by pericentric inversion, inv(10)(p12.1;q11.2), in papillary thyroid cancer from an atomic bomb survivor exposed to high-dose radiation". Oncology Reports 32.5 (2014): 1809-1814.
Chicago
Hamatani, K., Eguchi, H., Koyama, K., Mukai, M., Nakachi, K., Kusunoki, Y."A novel RET rearrangement (ACBD5/RET) by pericentric inversion, inv(10)(p12.1;q11.2), in papillary thyroid cancer from an atomic bomb survivor exposed to high-dose radiation". Oncology Reports 32, no. 5 (2014): 1809-1814. https://doi.org/10.3892/or.2014.3449