SPAG9 expression is increased in human prostate cancer and promotes cell motility, invasion and angiogenesis in vitro Corrigendum in /10.3892/or.2022.8314

Chen,Feifei; Lu,Zheng; Deng,Junpeng; Han,Xuechao; Bai,Jin; Liu,Qinghua; Xi,Yaguang; Zheng,Junnian

doi:10.3892/or.2014.3539

SPAG9 expression is increased in human prostate cancer and promotes cell motility, invasion and angiogenesis in vitro

Corrigendum in 10.3892/or.2022.8314

Authors:
- Feifei Chen
- Zheng Lu
- Junpeng Deng
- Xuechao Han
- Jin Bai
- Qinghua Liu
- Yaguang Xi
- Junnian Zheng
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Affiliations: Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China, School of Pathology, Xuzhou Medical College, Xuzhou, Jiangsu, P.R. China
Published online on: October 10, 2014 https://doi.org/10.3892/or.2014.3539
Pages: 2533-2540

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Abstract

Sperm-associated antigen 9 (SPAG9) is a recently characterized oncoprotein involved in the progression of several human malignancies. To elucidate the role of SPAG9 in the development of human prostate cancer (PCa), tissue microarray (TMA) and immunohistochemistry were used to detect the clinical relevance of SPAG9 in PCa tissues. We found that SPAG9 expression was increased in the PCa tissues when compared with the level in the tumor adjacent normal prostate tissues, and increased SPAG9 staining was significantly correlated with TNM stage and tumor grade. We also examined prostate cancer cell motility, invasion and angiogenesis ability following reduced SPAG9 expression by siRNA. Our data showed that knockdown of SPAG9 in prostate cancer cell lines inhibited cell motility and invasion due to the inactivation of metalloproteinase-2 (MMP‑2)/MMP-9 by upregulation of tissue inhibitor of metalloproteinase-1 (TIMP-1)/TIMP-2. Furthermore, downregulation of vascular endothelial growth factor (VEGF) secretion greatly contributed to the reduced ability of angiogenesis. Our data indicate that SPAG9 expression is significantly increased in PCa and it may be involved in the process of prostate cancer cell motility, migration and angiogenesis.

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