Overexpression of ZFX and its involvement in squamous cell carcinoma of the tongue

Yin,Jirong; Jiang,Yi; Wu,Hao; Wang,Juncheng; Zhang,Shanchuang; Liu,Hongchen

doi:10.3892/or.2014.3572

Overexpression of ZFX and its involvement in squamous cell carcinoma of the tongue

Authors:
- Jirong Yin
- Yi Jiang
- Hao Wu
- Juncheng Wang
- Shanchuang Zhang
- Hongchen Liu
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Affiliations: Dental Institute, Chinese PLA General Hospital, Beijing 100853, P.R. China
Published online on: October 29, 2014 https://doi.org/10.3892/or.2014.3572
Pages: 141-148

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Abstract

Zinc finger protein, X-linked (ZFX) has been identified as a transcriptional factor and is implicated in the development of variant types of cancer. Furthermore, it has been reported that ZFX is essential for the survival and self-renewal of embryonic stem cells. To investigate the involvement of ZFX in squamous cell carcinoma of the tongue, in the present study, we explored the expression of ZFX in clinical specimens from patients with squamous cell carcinoma of the tongue and the correlation between ZFX expression and multiple clinical pathological parameters. We further evaluated the impact of ZFX knockdown on the proliferation, colony formation ability, cell cycle distribution and survival of two human tongue squamous cell carcinoma cell lines to explore its critical role in the development of squamous cell carcinoma of the tongue. Our results showed that ZFX expression was aberrantly higher in samples from patients with squamous cell carcinoma of the tongue and revealed that ZFX expression is positively correlated with tumor grade and stage. Consistent with these findings, we further found that ZFX knockdown impaired cell proliferation and colony formation ability and induced cell apoptosis and cell cycle arrest in two human tongue squamous cell carcinoma cell lines. Our results indicate that ZFX is essential for the development and progression of squamous cell carcinoma of the tongue and represents a potential target for the development of effective therapy.

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1	Warnakulasuriya S: Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 45:309–316. 2009. View Article : Google Scholar
2	Ganly I, Patel S and Shah J: Early stage squamous cell cancer of the oral tongue - clinicopathologic features affecting outcome. Cancer. 118:101–111. 2012. View Article : Google Scholar
3	Galan-Caridad JM, Harel S, Arenzana TL, et al: Zfx controls the self-renewal of embryonic and hematopoietic stem cells. Cell. 129:345–357. 2007. View Article : Google Scholar : PubMed/NCBI
4	Weisberg SP, Smith-Raska MR, Esquilin JM, et al: ZFX controls propagation and prevents differentiation of acute T-lymphoblastic and myeloid leukemia. Cell Rep. 6:528–540. 2014. View Article : Google Scholar : PubMed/NCBI
5	Haapa-Paananen S, Kiviluoto S, Waltari M, et al: HES6 gene is selectively overexpressed in glioma and represents an important transcriptional regulator of glioma proliferation. Oncogene. 31:1299–1310. 2012. View Article : Google Scholar
6	Wang L, Zhou GB, Liu P, et al: Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia. Proc Natl Acad Sci USA. 105:4826–4831. 2008. View Article : Google Scholar : PubMed/NCBI
7	Sasaki K, Tsuno NH, Sunami E, et al: Chloroquine potentiates the anti-cancer effect of 5-fluorouracil on colon cancer cells. BMC Cancer. 10:3702010. View Article : Google Scholar : PubMed/NCBI
8	Chen J, Xie F, Zhang L and Jiang WG: iASPP is over-expressed in human non-small cell lung cancer and regulates the proliferation of lung cancer cells through a p53 associated pathway. BMC Cancer. 10:6942010. View Article : Google Scholar
9	Hanahan D and Weinberg RA: Hallmarks of cancer: the next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI
10	Fernald K and Kurokawa M: Evading apoptosis in cancer. Trends Cell Biol. 23:620–633. 2013. View Article : Google Scholar : PubMed/NCBI
11	Chaturvedi AK, Anderson WF, Lortet-Tieulent J, et al: Worldwide trends in incidence rates for oral cavity and oropharyngeal cancers. J Clin Oncol. 31:4550–4559. 2013. View Article : Google Scholar : PubMed/NCBI
12	Fang J, Yu Z, Lian M, et al: Knockdown of zinc finger protein, X-linked (ZFX) inhibits cell proliferation and induces apoptosis in human laryngeal squamous cell carcinoma. Mol Cell Biochem. 360:301–307. 2012. View Article : Google Scholar
13	Jiang H, Zhang L, Liu J, et al: Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9. Cancer Gene Ther. 19:684–689. 2012. View Article : Google Scholar : PubMed/NCBI
14	Wu S, Lao XY, Sun TT, et al: Knockdown of ZFX inhibits gastric cancer cell growth in vitro and in vivo via downregulating the ERK-MAPK pathway. Cancer Lett. 337:293–300. 2013. View Article : Google Scholar : PubMed/NCBI
15	Akiyoshi S, Fukagawa T, Ueo H, et al: Clinical significance of miR-144-ZFX axis in disseminated tumour cells in bone marrow in gastric cancer cases. Br J Cancer. 107:1345–1353. 2012. View Article : Google Scholar : PubMed/NCBI
16	Nikpour P, Emadi-Baygi M, Mohammad-Hashem F, Maracy MR and Haghjooy-Javanmard S: Differential expression of ZFX gene in gastric cancer. J Biosci. 37:85–90. 2012. View Article : Google Scholar : PubMed/NCBI
17	Zhou Y, Su Z, Huang Y, et al: The Zfx gene is expressed in human gliomas and is important in the proliferation and apoptosis of the human malignant glioma cell line U251. J Exp Clin Cancer Res. 30:1142011. View Article : Google Scholar : PubMed/NCBI
18	Zhu Z, Li K, Xu D, et al: ZFX regulates glioma cell proliferation and survival in vitro and in vivo. J Neurooncol. 112:17–25. 2013. View Article : Google Scholar : PubMed/NCBI
19	Zha WJ, Cao L, Shen Y and Huang M: Roles of mir-144-ZFX pathway in growth regulation of non-small-cell lung cancer. PLoS One. 8:e741752013. View Article : Google Scholar : PubMed/NCBI
20	Yang H, Lu Y, Zheng Y, et al: shRNA-mediated silencing of ZFX attenuated the proliferation of breast cancer cells. Cancer Chemother Pharmacol. 73:569–576. 2014. View Article : Google Scholar : PubMed/NCBI
21	Li K, Zhu ZC, Liu YJ, et al: ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299. Int J Clin Exp Pathol. 6:2460–2467. 2013.PubMed/NCBI