Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells

  • Authors:
    • Hak-Su Kim
    • Jihyun Lim
    • Da Yeon Lee
    • Jae-Ha Ryu
    • Jong-Seok Lim
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  • Published online on: November 12, 2014     https://doi.org/10.3892/or.2014.3601
  • Pages: 223-229
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Abstract

Kazinol C is a 1,3-diphenylpropane, obtained from Broussonetia kazinoki, that has been employed in folk medicine as an edema suppressant. It exerts beneficial effects in oxidative stress-related diseases, such as cancer. However, the molecular mechanism involved in the anticancer effects remains to be determined. AMP-activated protein kinase (AMPK) has emerged as a possible anticancer target molecule. The present study investigated the effect of kazinol C on AMPK activation as well as subsequent HT-29 colon cancer cell viability, apoptosis and migration. Kazinol C markedly induced AMPK phosphorylation and significantly attenuated HT-29 colon cancer cell growth and viability. Compound C, as a well‑known AMPK inhibitor, blocked the kazinol C-induced cell death, and stable transduction of dominant-negative (DN) AMPK in colon cancer cells also inhibited kazinol C-induced cell apoptosis. In addition, kazinol C inhibited HT-29 cell migration and anchorage-independent growth. AMPK inhibition using stable transduction with DN AMPK significantly abrogated the kazinol C-induced inhibition of cancer cell migration. Thus, AMPK is a critical and novel regulator of kazinol C-mediated cancer cell apoptosis and inhibition of migration, suggesting that AMPK is a prime cancer target.
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January-2015
Volume 33 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kim H, Lim J, Lee DY, Ryu J and Lim J: Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncol Rep 33: 223-229, 2015.
APA
Kim, H., Lim, J., Lee, D.Y., Ryu, J., & Lim, J. (2015). Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells. Oncology Reports, 33, 223-229. https://doi.org/10.3892/or.2014.3601
MLA
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33.1 (2015): 223-229.
Chicago
Kim, H., Lim, J., Lee, D. Y., Ryu, J., Lim, J."Kazinol C from Broussonetia kazinoki activates AMP-activated protein kinase to induce antitumorigenic effects in HT-29 colon cancer cells". Oncology Reports 33, no. 1 (2015): 223-229. https://doi.org/10.3892/or.2014.3601