Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer

  • Authors:
    • Ningning Cheng
    • Xuefei Li
    • Chao Zhao
    • Shengxiang Ren
    • Xiaoxia Chen
    • Weijing Cai
    • Mingchuan Zhao
    • Yishi Zhang
    • Jiayu Li
    • Qi Wang
    • Caicun Zhou
  • View Affiliations

  • Published online on: December 2, 2014     https://doi.org/10.3892/or.2014.3643
  • Pages: 833-839
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Abstract

The application of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) is limited by drug resistance in non-small cell lung cancer (NSCLC). Long non-coding RNAs (lncRNAs) are known to be involved in tumor development and metastasis, as well as chemotherapy resistance. To gain insight into the molecular mechanisms of EGFR-TKIs resistance, EGFR-TKIs‑sensitive and ‑resistant human lung cancer cells were analyzed by lncRNA microarray. In the present study, we found a total of 22,587 lncRNAs expressed in lung cancer cells. Of these, the expression level of 1,731 lncRNAs was upregulated >2-fold compared with gefitinib-sensitive cells while that of 2,936 was downregulated. Bioinformatics analysis (GO and pathway analyses) revealed that some classical pathways participating in cell proliferation and apoptosis were aberrantly expressed in these cells (P-value cut-off was 0.05). Enhancer-like lncRNAs and their nearby coding genes were analyzed. Six lncRNAs were identified as potential enhancers. Several lncRNAs were validated in lung cancer cell lines using RT-qPCR. To the best of our knowledge, the results showed for the first time that differentially expressed lncRNAs responded to EGFR-TKIs resistance in NSCLC cells. LncRNAs may therefore be novel candidate biomarkers and potential targets for EGFR-TKIs therapy in the future.
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February-2015
Volume 33 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cheng N, Li X, Zhao C, Ren S, Chen X, Cai W, Zhao M, Zhang Y, Li J, Wang Q, Wang Q, et al: Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer. Oncol Rep 33: 833-839, 2015.
APA
Cheng, N., Li, X., Zhao, C., Ren, S., Chen, X., Cai, W. ... Zhou, C. (2015). Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer. Oncology Reports, 33, 833-839. https://doi.org/10.3892/or.2014.3643
MLA
Cheng, N., Li, X., Zhao, C., Ren, S., Chen, X., Cai, W., Zhao, M., Zhang, Y., Li, J., Wang, Q., Zhou, C."Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer". Oncology Reports 33.2 (2015): 833-839.
Chicago
Cheng, N., Li, X., Zhao, C., Ren, S., Chen, X., Cai, W., Zhao, M., Zhang, Y., Li, J., Wang, Q., Zhou, C."Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer". Oncology Reports 33, no. 2 (2015): 833-839. https://doi.org/10.3892/or.2014.3643