Microarray analysis of the aberrant microRNA expression pattern in gliomas of different grades

Zhu,Xiao-Peng; Mou,Ke-Jie; Xu,Qing-Fu; Tang,Jun-Hai; Huang,Guo-Hao; Xu,Jian-Ping; Li,Guang-Hui; Ai,Si-Jin; Hugnot,Jean-Phillippe; Zhou,Zheng; Lv,Sheng-Qing

doi:10.3892/or.2015.3953

Microarray analysis of the aberrant microRNA expression pattern in gliomas of different grades

Authors:
- Xiao-Peng Zhu
- Ke-Jie Mou
- Qing-Fu Xu
- Jun-Hai Tang
- Guo-Hao Huang
- Jian-Ping Xu
- Guang-Hui Li
- Si-Jin Ai
- Jean-Phillippe Hugnot
- Zheng Zhou
- Sheng-Qing Lv
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Affiliations: Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Pathology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Institute of Cancer, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China, Department of Neurosurgery, China People's Liberation Army No. 94 Hospital, Nanchang, Jiangxi 330026, P.R. China, INSERM U1051, Institut des Neurosciences de Montpellier, 34091 Montpellier, France
Published online on: May 6, 2015 https://doi.org/10.3892/or.2015.3953
Pages: 318-324

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Abstract

Previous studies have focused on miRNA expression in brain gliomas. However, both the expression pattern of miRNAs in gliomas of different grades and various miRNAs involved in malignant progression of gliomas are poorly understood. In the present study, we used miRNA microarray-based screening to investigate the miRNA expression profile in gliomas, which was further verified by qRT-PCR in selected miRNAs. In total, we found 13 differentially expressed miRNAs between gliomas and their matched surrounding tissues. Among them, 12 miRNAs were upregulated and only one (miR-4489) was downregulated compared with the control. Furthermore, the lower expression level of miR-4489 was confirmed by qRT-PCR in 26 glioma samples. Our microarray result revealed 8, 9 and 15 aberrantly expressed miRNAs in gliomas of World Health Organization (WHO) grade II-IV, respectively. Gene Ontology (GO) and Pathway analysis indicated that target genes of the 13 miRNAs were significantly enriched in central nervous system- and tumor‑related biological processes and signaling pathways. The dysregulated miRNAs identified in the present study contribute to the tumorigenesis and malignant progression of gliomas and may serve as useful markers for advanced glioma pathological grading and prognosis.

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