MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4

Chen,Tianjun; Gao,Fei; Feng,Sifang; Yang,Tian; Chen,Mingwei

doi:10.3892/or.2015.3978

MicroRNA-370 inhibits the progression of non-small cell lung cancer by downregulating oncogene TRAF4

Authors:
- Tianjun Chen
- Fei Gao
- Sifang Feng
- Tian Yang
- Mingwei Chen
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Affiliations: Respiratory Department, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China, Ultrasound Department, Hua-shan Central Hospital of Xi'an, Xi'an, Shaanxi, P.R. China
Published online on: May 13, 2015 https://doi.org/10.3892/or.2015.3978
Pages: 461-468

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Abstract

Lung cancer is the leading cause of cancer-related deaths, of which most can be attributed to non-small cell lung cancer (NSCLC). microRNAs (miRNAs) are a group of small non-coding RNAs that focus on post-transcriptional modification. The present study aimed to investigate the role and function of microRNA-370 (miR-370) in NSCLC and explore the underlying functional mechanisms. We found that miR-370 was significantly downregulated in the tumor tissues of NSCLC patients as well as in NSCLC cell lines. Overexpression of miR-370 by infection of recombinant lentivirus markedly inhibited cell proliferation and promoted cell apoptosis of NSCLC cells. In addition, in vivo tumor formation of NSCLC cells was decreased by miR-370 overexpression. Through bioinformatic analysis, we found that tumor necrosis factor receptor-associated factor 4 (TRAF4), an oncogene as previously reported, was predicted as a putative target gene of miR-370. The direct targeting relationship between miR-370 and the 3'-untranslated region was validated by dual-luciferase reporter assay. Furthermore, overexpression of miR-370 downregulated the protein expression of TRAF4 in the NSCLC cells. Moreover, the growth inhibitory effect of miR-370 overexpression on NSCLC cells was abrogated by TRAF4 overexpression. In conclusion, our results suggest that miR-370 plays an important role in NSCLC by regulating TRAF4 and may be a potential target for the treatment of NSCLC.

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