Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells

Huang,Yuhui; Kumazoe,Motofumi; Bae,Jaehoon; Yamada,Shuhei; Takai,Mika; Hidaka,Shiori; Yamashita,Shuya; Kim,Yoonhee; Won,Yeongseon; Murata,Motoki; Tsukamoto,Shuntaro; Tachibana,Hirofumi

doi:10.3892/or.2015.4086

Green tea polyphenol epigallocatechin-O-gallate induces cell death by acid sphingomyelinase activation in chronic myeloid leukemia cells

Authors:
- Yuhui Huang
- Motofumi Kumazoe
- Jaehoon Bae
- Shuhei Yamada
- Mika Takai
- Shiori Hidaka
- Shuya Yamashita
- Yoonhee Kim
- Yeongseon Won
- Motoki Murata
- Shuntaro Tsukamoto
- Hirofumi Tachibana
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Affiliations: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan
Published online on: June 26, 2015 https://doi.org/10.3892/or.2015.4086
Pages: 1162-1168
Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

An epidemiological study showed that green tea consumption is associated with a reduced risk of hematopoietic malignancy. The major green tea polyphenol epigallocatechin‑3-O-gallate (EGCG) is reported to have anticancer effects. Chronic myeloid leukemia (CML) is a major hematopoietic malignancy characterized by expansion of myeloid cells. In the present study, we showed EGCG-induced acid sphingomyelinase (ASM) activation and lipid raft clustering in CML cells. The ASM inhibitor desipramine significantly reduced EGCG-induced cell death. Protein kinase Cδ is a well‑known kinase that plays an important role in ASM activation. We observed EGCG-induced phosphorylation of protein kinase Cδ at Ser664. Importantly, EGCG-induced ASM activation was significantly reduced by pretreatment of CML cells with the soluble guanylate cyclase inhibitor NS2028, suggesting that EGCG induced ASM activation through the cyclic guanosine monophosphate (cGMP)-dependent pathway. Indeed, pharmacological inhibition of a cGMP-negative regulator enhanced the anti-CML effect of EGCG. These results indicate that EGCG-induced cell death via the cGMP/ASM pathway in CML cells.

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