Open Access

Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway

  • Authors:
    • Xiaolong Yu
    • Xin Zhou
    • Changlin Fu
    • Qiang Wang
    • Tao Nie
    • Fan Zou
    • Runsheng Guo
    • Hucheng Liu
    • Bin Zhang
    • Min Dai
  • View Affiliations

  • Published online on: July 13, 2015     https://doi.org/10.3892/or.2015.4124
  • Pages: 1129-1136
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Celastrol is an active compound extracted from the root bark of Triptergium wilfordii Hook F., also known as ̔Thunder of God Vine̓. It is a well-known Chinese medicinal herb that was found to inhibit tumor cell growth and promote apoptosis in several tumor cell lines. However, research into its effects on osteosarcoma cell apoptosis is still extremely limited. The present study was undertaken to determine the effect of celastrol on viability and apoptosis of osteosarcoma cells and furthermore, to illuminate the molecular mechanism of celastrol-induced osteosarcoma cell apoptosis. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay was used to evaluate the viability of the cells following treatment with celastrol. The effect of celastrol on the apoptotic rate of the cells was evaluated by flow cytometry using Annexin V-PE/7-AAD staining assay. Fluorescence microscopy was used to detect the morphological changes in the human osteosarcoma U-2OS cell lines. The expression of Bcl-2 family proteins, caspase-3, caspase-8, caspase-9, cytochrome c and PARP was measured by western blotting. We found that celastrol significantly inhibited the growth of osteosarcoma cells in a dose-dependent manner, particularly U-2OS cells. Furthermore, we observed that celastrol upregulated the expression of the pro-apoptotic proteins Bax and cytochrome c and altered the ratio of Bax/Bcl-2, and triggered the mitochondrial apoptotic pathway, resulting in caspase-3 and -9 activation and PARP cleavage. To conclude, the results indicate that celastrol inhibits the proliferation of human osteosarcoma cancer cells by inducing apoptosis via the mitochondrial-dependent pathway.
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September-2015
Volume 34 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yu X, Zhou X, Fu C, Wang Q, Nie T, Zou F, Guo R, Liu H, Zhang B, Dai M, Dai M, et al: Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway. Oncol Rep 34: 1129-1136, 2015.
APA
Yu, X., Zhou, X., Fu, C., Wang, Q., Nie, T., Zou, F. ... Dai, M. (2015). Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway. Oncology Reports, 34, 1129-1136. https://doi.org/10.3892/or.2015.4124
MLA
Yu, X., Zhou, X., Fu, C., Wang, Q., Nie, T., Zou, F., Guo, R., Liu, H., Zhang, B., Dai, M."Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway". Oncology Reports 34.3 (2015): 1129-1136.
Chicago
Yu, X., Zhou, X., Fu, C., Wang, Q., Nie, T., Zou, F., Guo, R., Liu, H., Zhang, B., Dai, M."Celastrol induces apoptosis of human osteosarcoma cells via the mitochondrial apoptotic pathway". Oncology Reports 34, no. 3 (2015): 1129-1136. https://doi.org/10.3892/or.2015.4124