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miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer

  • Authors:
    • Chun Xu
    • Sen Li
    • Tengfei Chen
    • Haibo Hu
    • Cheng Ding
    • Zhenlei Xu
    • Jun Chen
    • Zeyi Liu
    • Zhe Lei
    • Hong-Tao Zhang
    • Chang Li
    • Jun Zhao

  • View Affiliations

  • Published online on: November 4, 2015     https://doi.org/10.3892/or.2015.4392
  • Pages: 497-503
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
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Abstract

Polo-like kinase 1 (PLK1), a critical kinase for mitotic progression, is overexpressed in a wide range of cancers. MicroRNAs (miRNAs) are a class of small non-coding RNA molecules and proposed to play important roles in the regulation of tumor progression and invasion. However, the relationship between PLK1 and miRNAs have remained unclear. In the present study, the association between PLK1 and miR-296-5p was investigated. The upregulation of PLK1 mRNA expression levels combined with the downregulation of miR-296-5p levels were detected in both non-small cell lung cancer (NSCLC) tissues and cell lines. Functional studies showed that knockdown of PLK1 by siRNA inhibited NSCLC cells proliferation. Impressively, overexpression of miR-296-5p showed the same phenocopy as the effect of PLK1 knockdown in NSCLC cells, indicating that PLK1 was a major target of miR-296-5p. Furthermore, using western blot analysis and luciferase reporter assay, PLK1 protein expression was proved to be regulated by miR-296-5p through binding to the putative binding sites in its 3'-untranslated region (3'-UTR). Taken together, the present study indicated that miR-296-5p regulated PLK1 expression and could function as a tumor suppressor in NSCLC progression, which provides a potential target for gene therapy of NSCLC.
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January-2016
Volume 35 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Xu C, Li S, Chen T, Hu H, Ding C, Xu Z, Chen J, Liu Z, Lei Z, Zhang H, Zhang H, et al: miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer. Oncol Rep 35: 497-503, 2016.
APA
Xu, C., Li, S., Chen, T., Hu, H., Ding, C., Xu, Z. ... Zhao, J. (2016). miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer. Oncology Reports, 35, 497-503. https://doi.org/10.3892/or.2015.4392
MLA
Xu, C., Li, S., Chen, T., Hu, H., Ding, C., Xu, Z., Chen, J., Liu, Z., Lei, Z., Zhang, H., Li, C., Zhao, J."miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer". Oncology Reports 35.1 (2016): 497-503.
Chicago
Xu, C., Li, S., Chen, T., Hu, H., Ding, C., Xu, Z., Chen, J., Liu, Z., Lei, Z., Zhang, H., Li, C., Zhao, J."miR-296-5p suppresses cell viability by directly targeting PLK1 in non-small cell lung cancer". Oncology Reports 35, no. 1 (2016): 497-503. https://doi.org/10.3892/or.2015.4392