search button

Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9

  • Authors:
    • Min Xiong
    • Lin Wang
    • Hua‑Long Yu
    • Heng Han
    • Dan Mao
    • Jie Chen
    • Yun Zeng
    • Ning He
    • Zhi‑Gang Liu
    • Zhi‑Yong Wang
    • Shou‑Jia Xu
    • Le-Yun Guo
    • Yong-An Wang

  • View Affiliations

  • Published online on: November 16, 2015     https://doi.org/10.3892/or.2015.4427
  • Pages: 1034-1040
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Osteosarcoma is composed of tumor osteoblasts and bone-like tissues, with malignant tumors originating from osteogenesis organization. Osteosarcoma is a primary malignant bone tumor. Invasion and metastasis of osteosarcoma affect the prognosis of patients. However, effective therapeutic treatments remain to be identified. The aim of the present study was to investigate the possible inhibitory and apoptotic effects of ginkgetin in osteosarcoma cells. 3.3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays were used to determine the effect ginkgetin exerted on the growth of osteosarcoma cells. Flow cytometry was used to determine cell apoptosis. STAT3 protein expression and activation of caspase-3/9 were measured using western blot analysis and the MTT and LDH assays, respectively. The results showed that ginkgetin inhibited cell growth and induced cell cytotoxicity in osteosarcoma cells in a dose-dependent manner. Treatment with ginkgetin significantly activated the apoptosis of osteosarcoma cells in a concentration-dependent manner. The anticancer activity of ginkgetin significantly inhibited STAT3 and promoted caspase-3/9 activation in osteosarcoma cells. The findings demonstrated that ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through the inhibition of STAT3 and activation of caspase-3/9.
View Figures
View References

Related Articles

Journal Cover

February-2016
Volume 35 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 3.8
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 8.5
  • CiteScore Rank: Q1: #74/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.719
  • SCImago Journal Rank (SJR): 0.864
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Xiong M, Wang L, Yu HL, Han H, Mao D, Chen J, Zeng Y, He N, Liu ZG, Wang ZY, Wang ZY, et al: Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncol Rep 35: 1034-1040, 2016.
APA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J. ... Wang, Y. (2016). Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9. Oncology Reports, 35, 1034-1040. https://doi.org/10.3892/or.2015.4427
MLA
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35.2 (2016): 1034-1040.
Chicago
Xiong, M., Wang, L., Yu, H., Han, H., Mao, D., Chen, J., Zeng, Y., He, N., Liu, Z., Wang, Z., Xu, S., Guo, L., Wang, Y."Ginkgetin exerts growth inhibitory and apoptotic effects on osteosarcoma cells through inhibition of STAT3 and activation of caspase-3/9". Oncology Reports 35, no. 2 (2016): 1034-1040. https://doi.org/10.3892/or.2015.4427