Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer

  • Authors:
    • Meng-Chi Yen
    • Ying-Chu Shih
    • Ya-Ling Hsu
    • En-Shyh Lin
    • Yi-Shiuan Lin
    • Eing-Mei Tsai
    • Ya-Wen Ho
    • Ming-Feng Hou
    • Po-Lin Kuo
  • View Affiliations

  • Published online on: December 22, 2015     https://doi.org/10.3892/or.2015.4503
  • Pages: 1356-1364
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Abstract

Development of an efficient treatment for triple-negative breast cancer is an urgent issues. Compounds from plant extracts are a potential source of novel cancer treatment. Isolinderalactone, a kind of sesquiterpenoids compound, was purified from the root of Lindera strychnifolia and Neolitsea daibuensis and shows anti-inflammatory and anticancer capacity. In the present study, isolinderalactone induced apoptosis in MDA-MB-231 cells which is a kind of triple-negative breast cancer cell line through induction of an intrinsic mitochondria-mediated and caspase-independent cell death. Treatment of isolinderalactone increased the protein level of the suppressor of cytokine signaling 3 (SCOS3), decreased phosphorylation of the signal transducer and activator of transcription 3 (STAT3), and suppressed expression of the down-stream genes of the X-linked inhibitor of apoptosis protein in MDA-MB-231 cells. Our results further showed that the level of SOCS3 expression was induced by isolinderalactone due to inhibiting the microRNA hsa-miR-30c-5p (miR-30c) expression. In addition, intraperitoneal injection of isolinderalactone induced apoptosis in a xenograft breast tumor while it did not significantly affect the histology of liver, kidney and lung of the treated mice. In conclusion, isolinderalactone induces apoptosis in MDA-MB‑231 cells and suppresses STAT3 signaling pathway through regulation of SOCS3 and miR-30c. It may become a novel treatment for triple-negative breast cancer in the future.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yen M, Shih Y, Hsu Y, Lin E, Lin Y, Tsai E, Ho Y, Hou M and Kuo P: Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer. Oncol Rep 35: 1356-1364, 2016.
APA
Yen, M., Shih, Y., Hsu, Y., Lin, E., Lin, Y., Tsai, E. ... Kuo, P. (2016). Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer. Oncology Reports, 35, 1356-1364. https://doi.org/10.3892/or.2015.4503
MLA
Yen, M., Shih, Y., Hsu, Y., Lin, E., Lin, Y., Tsai, E., Ho, Y., Hou, M., Kuo, P."Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer". Oncology Reports 35.3 (2016): 1356-1364.
Chicago
Yen, M., Shih, Y., Hsu, Y., Lin, E., Lin, Y., Tsai, E., Ho, Y., Hou, M., Kuo, P."Isolinderalactone enhances the inhibition of SOCS3 on STAT3 activity by decreasing miR-30c in breast cancer". Oncology Reports 35, no. 3 (2016): 1356-1364. https://doi.org/10.3892/or.2015.4503