Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo

Liu,Jin; Zhang,Ke; Zhen,Yong-Zhan; Wei,Jie; Hu,Gang; Gao,Jun-Ling; Tian,Yan-Xia; Lin,Ya-Jun

doi:10.3892/or.2015.4518

Antitumor activity of rhein lysinate against human glioma U87 cells in vitro and in vivo

Authors:
- Jin Liu
- Ke Zhang
- Yong-Zhan Zhen
- Jie Wei
- Gang Hu
- Jun-Ling Gao
- Yan-Xia Tian
- Ya-Jun Lin
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Affiliations: College of Life Sciences, Beijing Normal University, Beijing 100875, P.R. China, Oncology Department, Rizhao City People's Hospital, Rizhao, Shandong 276800, P.R. China, Key Laboratory for Chronic Diseases of Hebei Province, Key Laboratory for Preclinical and Basic Research on Chronic Diseases of Tangshan City, School of Basic Medical Sciences, North China University of Science and Technology, Tangshan, Hebei 063000, P.R. China, The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Chinese Ministry of Health, Beijing 100730, P.R. China
Published online on: December 24, 2015 https://doi.org/10.3892/or.2015.4518
Pages: 1711-1717

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Abstract

In previous studies, we demonstrated that rhein lysinate (RHL), the salt of rhein and lysine that is easily dissolved in water, inhibited the growth of tumor cells derived from breast and ovarian cancer, hepatocellular carcinoma, cervical cancer and lung carcinoma. Based on these observations, human glioma U87 cells and a xenograft model in BALB/c nude mice were used to examine the antitumor activity of RHL against human glioma. Notably, RHL statistically significantly suppressed the growth of human glioma U87 xenografts in BALB/c nude mice. In vitro, there was a significant reduction in cell proliferation after treatment with RHL in a dose- and time-dependent manner. The overall growth inhibition was correlated with the increase in reactive oxygen species (ROS) production and cell apoptosis. The apoptosis- and cell cycle-related proteins including BAX and Bim were increased, whereas Bcl-2 and cyclin D were decreased in the RHL-treated cells. The results demonstrated that RHL is highly effective against the growth of human glioma U87 xenografts in BALB/c nude mice. The potent antitumor activity of RHL may be mediated through downregulation of Bcl-2 and cyclin D expression and upregulation of BAX and Bim expression.

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