CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells

  • Authors:
    • Xiaowen Yu
    • Renping Zhao
    • Sensen Lin
    • Xianshu Bai
    • Luyong Zhang
    • Shengtao Yuan
    • Li Sun
  • View Affiliations

  • Published online on: December 24, 2015     https://doi.org/10.3892/or.2015.4520
  • Pages: 1557-1565
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Abstract

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a new angiogenic factor inducing angiogenesis via extracellular signal-regulated kinases pathway. To further understand the molecular mechanism underlying CXCL16‑induced angiogenesis, we explored involvement of other relevant pathways in CXCL16-induced angiogenesis. In the present study, we investigated the mechanisms underlying CXCL16-induced angiogenesis in human umbilical vein endothelial cells (HUVECs). CXCL16 promoted HUVEC proliferation, tube formation and migration. Enzyme-linked immunosorbent assay revealed that CXCL16 induced vascular endothelial growth factor secretion from HUVECs. Western blot analysis showed that CXCL16 increased the level of hypoxia‑inducible factor 1α, p-extracellular signal-regulated kinases (ERK), p-p38 and p-Akt dose- and time-dependently. ERK-, p38- and Akt-selective inhibitors significantly suppressed HUVEC proliferation, migration, tube formation and hypoxia-inducible factor 1α (HIF-1α) expression induced by CXCL16. Furthermore, CXCL16 peptides induced CXCL16 secretion via ERK, p38 and Akt pathways, which was suppressed by HIF-1α-selective inhibitor PX12. Our data suggest that CXCL16 induces angiogenesis in autocrine manner via ERK, Akt, p38 pathways and HIF-1α modulation.
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March-2016
Volume 35 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yu X, Zhao R, Lin S, Bai X, Zhang L, Yuan S and Sun L: CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells. Oncol Rep 35: 1557-1565, 2016.
APA
Yu, X., Zhao, R., Lin, S., Bai, X., Zhang, L., Yuan, S., & Sun, L. (2016). CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells. Oncology Reports, 35, 1557-1565. https://doi.org/10.3892/or.2015.4520
MLA
Yu, X., Zhao, R., Lin, S., Bai, X., Zhang, L., Yuan, S., Sun, L."CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells". Oncology Reports 35.3 (2016): 1557-1565.
Chicago
Yu, X., Zhao, R., Lin, S., Bai, X., Zhang, L., Yuan, S., Sun, L."CXCL16 induces angiogenesis in autocrine signaling pathway involving hypoxia-inducible factor 1α in human umbilical vein endothelial cells". Oncology Reports 35, no. 3 (2016): 1557-1565. https://doi.org/10.3892/or.2015.4520