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Article

Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway

  • Authors:
    • Ranran Kong
    • Jie Feng
    • Yuefeng Ma
    • Bin Zhou
    • Shaomin Li
    • Wei Zhang
    • Jiantao Jiang
    • Jin Zhang
    • Zhe Qiao
    • Ting Zhang
    • Quanjin Zang
    • Xijing He
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China, Department of Nephrology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Second Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 2306-2314
    |
    Published online on: January 13, 2016
       https://doi.org/10.3892/or.2016.4552
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Abstract

Non-small cell lung cancer (NSCLC) is the most common type of lung tumor with poor prognosis, in which the Notch signaling pathway plays an important role. Notch activation complex kinase (NACK) has been reported both as a co-activator and a target gene of the Notch pathway. However, the molecular mechanism of NACK in NSCLC still remains unknown. In this study, the expression of NACK was analyzed in 35 paired NSCLC tumor samples and 2 NSCLC cell lines. MTT assay, cell migration assay, cell invasion assay, flow cytometry assay, and xenograft model were employed to detect the effect of NACK knockdown on the cell proliferation, metastasis, invasion and apoptosis of NSCLC. The relationship between NACK and Notch1 signaling pathway in NSCLC cells was assessed by western blot and luciferase reporter assay. We found that the expression of NACK in the NSCLC tissues and lung cancer cells were significantly increased. High level of NACK expression is remarkable associated with tumor differentiation, lymphatic metastasis, clinical stage and poor survival prognosis. The interference of NACK significantly inhibited cell proliferation, invasion and metastasis through inducing apoptosis in NSCLC cells. The transcriptional activity of related Notch1 target genes were significantly suppressed resulting from NACK knockdown. This study demonstrates that interference of NACK inhibits NSCLC progression through Notch1 signaling pathway and targeting NACK may be an effective approach for NSCLC therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Kong R, Feng J, Ma Y, Zhou B, Li S, Zhang W, Jiang J, Zhang J, Qiao Z, Zhang T, Zhang T, et al: Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway. Oncol Rep 35: 2306-2314, 2016.
APA
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W. ... He, X. (2016). Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway. Oncology Reports, 35, 2306-2314. https://doi.org/10.3892/or.2016.4552
MLA
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, T., Zang, Q., He, X."Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway". Oncology Reports 35.4 (2016): 2306-2314.
Chicago
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, T., Zang, Q., He, X."Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway". Oncology Reports 35, no. 4 (2016): 2306-2314. https://doi.org/10.3892/or.2016.4552
Copy and paste a formatted citation
x
Spandidos Publications style
Kong R, Feng J, Ma Y, Zhou B, Li S, Zhang W, Jiang J, Zhang J, Qiao Z, Zhang T, Zhang T, et al: Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway. Oncol Rep 35: 2306-2314, 2016.
APA
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W. ... He, X. (2016). Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway. Oncology Reports, 35, 2306-2314. https://doi.org/10.3892/or.2016.4552
MLA
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, T., Zang, Q., He, X."Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway". Oncology Reports 35.4 (2016): 2306-2314.
Chicago
Kong, R., Feng, J., Ma, Y., Zhou, B., Li, S., Zhang, W., Jiang, J., Zhang, J., Qiao, Z., Zhang, T., Zang, Q., He, X."Silencing NACK by siRNA inhibits tumorigenesis in non-small cell lung cancer via targeting Notch1 signaling pathway". Oncology Reports 35, no. 4 (2016): 2306-2314. https://doi.org/10.3892/or.2016.4552
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