Real-time RT-PCR systems for CTC detection from blood samples of breast cancer and gynaecological tumour patients (Review)
- Authors:
- Ulrich Andergassen
- Alexandra C. Kölbl
- Sven Mahner
- Udo Jeschke
-
Affiliations: Department of Gynecology and Obstetrics, Ludwig-Maximilians-University of Munich, D-80337 Munich, Germany - Published online on: February 2, 2016 https://doi.org/10.3892/or.2016.4608
- Pages: 1905-1915
This article is mentioned in:
Abstract
Asworth T: A case of cancer in which cells similar to those in the tumours were seen in the blood after death. Australas Med J. 14:146–147. 1869. | |
Paget S: The distribution of secondary growths in cancer of the breast. 1889. Cancer Metastasis Rev. 8:98–101. 1989.PubMed/NCBI | |
Fidler IJ: The pathogenesis of cancer metastasis: The 'seed and soil' hypothesis revisited. Nat Rev Cancer. 3:453–458. 2003. View Article : Google Scholar : PubMed/NCBI | |
Tibbe AG, Miller MC and Terstappen LW: Statistical considerations for enumeration of circulating tumor cells. Cytometry A. 71:154–162. 2007. View Article : Google Scholar : PubMed/NCBI | |
Park Y, Kitahara T, Urita T, Yoshida Y and Kato R: Expected clinical applications of circulating tumor cells in breast cancer. World J Clin Oncol. 2:303–310. 2011. View Article : Google Scholar : PubMed/NCBI | |
Balic M, Lin H, Williams A, Datar RH and Cote RJ: Progress in circulating tumor cell capture and analysis: Implications for cancer management. Expert Rev Mol Diagn. 12:303–312. 2012. View Article : Google Scholar : PubMed/NCBI | |
Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, Tibbe AG, Uhr JW and Terstappen LW: Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res. 10:6897–6904. 2004. View Article : Google Scholar : PubMed/NCBI | |
Davis JW, Nakanishi H, Kumar VS, Bhadkamkar VA, McCormack R, Fritsche HA, Handy B, Gornet T and Babaian RJ: Circulating tumor cells in peripheral blood samples from patients with increased serum prostate specific antigen: Initial results in early prostate cancer. J Urol. 179:2187–2191; discussion 2191. 2008. View Article : Google Scholar : PubMed/NCBI | |
Braun S, Vogl FD, Naume B, Janni W, Osborne MP, Coombes RC, Schlimok G, Diel IJ, Gerber B, Gebauer G, et al: A pooled analysis of bone marrow micrometastasis in breast cancer. N Engl J Med. 353:793–802. 2005. View Article : Google Scholar : PubMed/NCBI | |
Reinholz MM, Nibbe A, Jonart LM, Kitzmann K, Suman VJ, Ingle JN, Houghton R, Zehentner B, Roche PC and Lingle WL: Evaluation of a panel of tumor markers for molecular detection of circulating cancer cells in women with suspected breast cancer. Clin Cancer Res. 11:3722–3732. 2005. View Article : Google Scholar : PubMed/NCBI | |
Zieglschmid V, Hollmann C and Böcher O: Detection of disseminated tumor cells in peripheral blood. Crit Rev Clin Lab Sci. 42:155–196. 2005. View Article : Google Scholar : PubMed/NCBI | |
Parsons HM, Tuttle TM, Kuntz KM, Begun JW, McGovern PM and Virnig BA: Association between lymph node evaluation for colon cancer and node positivity over the past 20 years. JAMA. 306:1089–1097. 2011. View Article : Google Scholar : PubMed/NCBI | |
Hermanek P, Hutter RV, Sobin LH and Wittekind C: International Union Against Cancer. Classification of isolated tumor cells and micrometastasis. Cancer. 86:2668–2673. 1999. View Article : Google Scholar : PubMed/NCBI | |
Singletary SE, Patel-Parekh L and Bland KI: Treatment trends in early-stage invasive lobular carcinoma: A report from the National Cancer Data Base. Ann Surg. 242:281–289. 2005. View Article : Google Scholar : PubMed/NCBI | |
Broersen LH, van Pelt GW, Tollenaar RA and Mesker WE: Clinical application of circulating tumor cells in breast cancer. Cell Oncol (Dordr). 37:9–15. 2014. View Article : Google Scholar | |
Fehm T, Hoffmann O, Aktas B, Becker S, Solomayer EF, Wallwiener D, Kimmig R and Kasimir-Bauer S: Detection and characterization of circulating tumor cells in blood of primary breast cancer patients by RT-PCR and comparison to status of bone marrow disseminated cells. Breast Cancer Res. 11:R592009. View Article : Google Scholar : PubMed/NCBI | |
McShane LM, Hunsberger S and Adjei AA: Effective incorporation of biomarkers into phase II trials. Clin Cancer Res. 15:1898–1905. 2009. View Article : Google Scholar : PubMed/NCBI | |
Pantel K and Alix-Panabières C: Circulating tumour cells in cancer patients: Challenges and perspectives. Trends Mol Med. 16:398–406. 2010. View Article : Google Scholar : PubMed/NCBI | |
García-Sáenz JA, Martín M, Maestro ML, Vidaurreta M, Veganzones S, Rafael S, Casado A, Bobokova J, Sastre J, De la Orden V, et al: Circulating tumour cells in locally advanced breast cancer. Clin Transl Oncol. 11:544–547. 2009. View Article : Google Scholar : PubMed/NCBI | |
Lianidou ES and Markou A: Circulating tumor cells as emerging tumor biomarkers in breast cancer. Clin Chem Lab Med. 49:1579–1590. 2011. View Article : Google Scholar : PubMed/NCBI | |
Fehm T, Müller V, Alix-Panabières C and Pantel K: Micrometastatic spread in breast cancer: Detection, molecular characterization and clinical relevance. Breast Cancer Res. 10(Suppl 1): S12008. View Article : Google Scholar : | |
Lianidou ES and Markou A: Molecular assays for the detection and characterization of CTCs. Recent Results Cancer Res. 195:111–123. 2012. View Article : Google Scholar : PubMed/NCBI | |
Pantel K and Alix-Panabières C: Detection methods of circulating tumor cells. J Thorac Dis. 4:446–447. 2012.PubMed/NCBI | |
Pantel K and Brakenhoff RH: Dissecting the metastatic cascade. Nat Rev Cancer. 4:448–456. 2004. View Article : Google Scholar : PubMed/NCBI | |
Uhr JW, Huebschman ML, Frenkel EP, Lane NL, Ashfaq R, Liu H, Rana DR, Cheng L, Lin AT, Hughes GA, et al: Molecular profiling of individual tumor cells by hyperspectral microscopic imaging. Transl Res. 159:366–375. 2012. View Article : Google Scholar : PubMed/NCBI | |
Pantel K, Alix-Panabières C and Riethdorf S: Cancer micrometastases. Nat Rev Clin Oncol. 6:339–351. 2009. View Article : Google Scholar : PubMed/NCBI | |
Geiger TM and Ricciardi R: Screening options and recommendations for colorectal cancer. Clin Colon Rectal Surg. 22:209–217. 2009. View Article : Google Scholar : | |
Negin BP and Cohen SJ: Circulating tumor cells in colorectal cancer: Past, present, and future challenges. Curr Treat Options Oncol. 11:1–13. 2010. View Article : Google Scholar : PubMed/NCBI | |
Kim MY, Oskarsson T, Acharyya S, Nguyen DX, Zhang XH, Norton L and Massagué J: Tumor self-seeding by circulating cancer cells. Cell. 139:1315–1326. 2009. View Article : Google Scholar | |
Hayes DC, Secrist H, Bangur CS, Wang T, Zhang X, Harlan D, Goodman GE, Houghton RL, Persing DH and Zehentner BK: Multigene real-time PCR detection of circulating tumor cells in peripheral blood of lung cancer patients. Anticancer Res. 26(2B): 1567–1575. 2006.PubMed/NCBI | |
Saloustros E and Mavroudis D: CTCs in primary breast cancer (II). Recent Results Cancer Res. 195:187–192. 2012. View Article : Google Scholar : PubMed/NCBI | |
Stathopoulou A, Gizi A, Perraki M, Apostolaki S, Malamos N, Mavroudis D, Georgoulias V and Lianidou ES: Real-time quantification of CK-19 mRNA-positive cells in peripheral blood of breast cancer patients using the lightcycler system. Clin Cancer Res. 9:5145–5151. 2003.PubMed/NCBI | |
Schoenfeld A, Kruger KH, Gomm J, Sinnett HD, Gazet JC, Sacks N, Bender HG, Luqmani Y and Coombes RC: The detection of micrometastases in the peripheral blood and bone marrow of patients with breast cancer using immunohistochemistry and reverse transcriptase polymerase chain reaction for keratin 19. Eur J Cancer. 33:854–861. 1997. View Article : Google Scholar : PubMed/NCBI | |
Ignatiadis M, Xenidis N, Perraki M, Apostolaki S, Politaki E, Kafousi M, Stathopoulos EN, Stathopoulou A, Lianidou E, Chlouverakis G, et al: Different prognostic value of cyto-keratin-19 mRNA positive circulating tumor cells according to estrogen receptor and HER2 status in early-stage breast cancer. J Clin Oncol. 25:5194–5202. 2007. View Article : Google Scholar : PubMed/NCBI | |
Xenidis N, Perraki M, Kafousi M, Apostolaki S, Bolonaki I, Stathopoulou A, Kalbakis K, Androulakis N, Kouroussis C, Pallis T, et al: Predictive and prognostic value of peripheral blood cytokeratin-19 mRNA-positive cells detected by real-time polymerase chain reaction in node-negative breast cancer patients. J Clin Oncol. 24:3756–3762. 2006. View Article : Google Scholar : PubMed/NCBI | |
Xenidis N, Ignatiadis M, Apostolaki S, Perraki M, Kalbakis K, Agelaki S, Stathopoulos EN, Chlouverakis G, Lianidou E, Kakolyris S, et al: Cytokeratin-19 mRNA-positive circulating tumor cells after adjuvant chemotherapy in patients with early breast cancer. J Clin Oncol. 27:2177–2184. 2009. View Article : Google Scholar : PubMed/NCBI | |
Sotiriou C and Pusztai L: Gene-expression signatures in breast cancer. N Engl J Med. 360:790–800. 2009. View Article : Google Scholar : PubMed/NCBI | |
Xenidis N, Markos V, Apostolaki S, Perraki M, Pallis A, Sfakiotaki G, Papadatos-Pastos D, Kalmanti L, Kafousi M, Stathopoulos E, et al: Clinical relevance of circulating CK-19 mRNA-positive cells detected during the adjuvant tamoxifen treatment in patients with early breast cancer. Ann Oncol. 18:1623–1631. 2007. View Article : Google Scholar : PubMed/NCBI | |
Saloustros E, Perraki M, Apostolaki S, Kallergi G, Xyrafas A, Kalbakis K, Agelaki S, Kalykaki A, Georgoulias V and Mavroudis D: Cytokeratin-19 mRNA-positive circulating tumor cells during follow-up of patients with operable breast cancer: Prognostic relevance for late relapse. Breast Cancer Res. 13:R602011. View Article : Google Scholar : PubMed/NCBI | |
Alix-Panabières C and Pantel K: Circulating tumor cells: Liquid biopsy of cancer. Clin Chem. 59:110–118. 2013. View Article : Google Scholar | |
Saucedo-Zeni N, Mewes S, Niestroj R, Gasiorowski L, Murawa D, Nowaczyk P, Tomasi T, Weber E, Dworacki G, Morgenthaler NG, et al: A novel method for the in vivo isolation of circulating tumor cells from peripheral blood of cancer patients using a functionalized and structured medical wire. Int J Oncol. 41:1241–1250. 2012.PubMed/NCBI | |
Sleijfer S, Gratama JW, Sieuwerts AM, Kraan J, Martens JW and Foekens JA: Circulating tumour cell detection on its way to routine diagnostic implementation? Eur J Cancer. 43:2645–2650. 2007. View Article : Google Scholar : PubMed/NCBI | |
Fehm T, Solomayer EF, Meng S, Tucker T, Lane N, Wang J and Gebauer G: Methods for isolating circulating epithelial cells and criteria for their classification as carcinoma cells. Cytotherapy. 7:171–185. 2005. View Article : Google Scholar : PubMed/NCBI | |
Königsberg R, Obermayr E, Bises G, Pfeiler G, Gneist M, Wrba F, de Santis M, Zeillinger R, Hudec M and Dittrich C: Detection of EpCAM positive and negative circulating tumor cells in meta-static breast cancer patients. Acta Oncol. 50:700–710. 2011. View Article : Google Scholar | |
Sieuwerts AM, Kraan J, Bolt-de Vries J, van der Spoel P, Mostert B, Martens JW, Gratama JW, Sleijfer S and Foekens JA: Molecular characterization of circulating tumor cells in large quantities of contaminating leukocytes by a multiplex real-time PCR. Breast Cancer Res Treat. 118:455–468. 2009. View Article : Google Scholar | |
Schindlbeck C, Stellwagen J, Jeschke U, Karsten U, Rack B, Janni W, Jückstock J, Tulusan A, Sommer H and Friese K: Immunomagnetic enrichment of disseminated tumor cells in bone marrow and blood of breast cancer patients by the Thomsen-Friedenreich-Antigen. Clin Exp Metastasis. 25:233–240. 2008. View Article : Google Scholar : PubMed/NCBI | |
Vona G, Sabile A, Louha M, Sitruk V, Romana S, Schütze K, Capron F, Franco D, Pazzagli M, Vekemans M, et al: Isolation by size of epithelial tumor cells : A new method for the immunomorphological and molecular characterization of circulatingtumor cells. Am J Pathol. 156:57–63. 2000. View Article : Google Scholar : PubMed/NCBI | |
Busch R, Cesar D, Higuera-Alhino D, Gee T, Hellerstein MK and McCune JM: Isolation of peripheral blood CD4+ T cells using RosetteSep and MACS for studies of DNA turnover by deuterium labeling. J Immunol Methods. 286:97–109. 2004. View Article : Google Scholar : PubMed/NCBI | |
Hayes DF and Smerage JB: Circulating tumor cells. Prog Mol Biol Transl Sci. 95:95–112. 2010. View Article : Google Scholar : PubMed/NCBI | |
Riethdorf S, Fritsche H, Müller V, Rau T, Schindlbeck C, Rack B, Janni W, Coith C, Beck K, Jänicke F, et al: Detection of circulating tumor cells in peripheral blood of patients with metastatic breast cancer: A validation study of the CellSearch system. Clin Cancer Res. 13:920–928. 2007. View Article : Google Scholar : PubMed/NCBI | |
Cristofanilli M: The biological information obtainable from circulating tumor cells. Breast. 18(Suppl 3): S38–S40. 2009. View Article : Google Scholar : PubMed/NCBI | |
Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, Reuben JM, Doyle GV, Allard WJ, Terstappen LW, et al: Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N Engl J Med. 351:781–791. 2004. View Article : Google Scholar : PubMed/NCBI | |
Nolé F, Munzone E, Zorzino L, Minchella I, Salvatici M, Botteri E, Medici M, Verri E, Adamoli L, Rotmensz N, et al: Variation of circulating tumor cell levels during treatment of metastatic breast cancer: Prognostic and therapeutic implications. Ann Oncol. 19:891–897. 2008. View Article : Google Scholar | |
Bidard FC, Mathiot C, Delaloge S, Brain E, Giachetti S, de Cremoux P, Marty M and Pierga JY: Single circulating tumor cell detection and overall survival in nonmetastatic breast cancer. Ann Oncol. 21:729–733. 2010. View Article : Google Scholar | |
Botteri E, Sandri MT, Bagnardi V, Munzone E, Zorzino L, Rotmensz N, Casadio C, Cassatella MC, Esposito A, Curigliano G, et al: Modeling the relationship between circulating tumour cells number and prognosis of metastatic breast cancer. Breast Cancer Res Treat. 122:211–217. 2010. View Article : Google Scholar | |
Jakob C, Aust DE, Liebscher B, Baretton GB, Datta K and Muders MH: Lymphangiogenesis in regional lymph nodes is an independent prognostic marker in rectal cancer patients after neoadjuvant treatment. PLoS One. 6:e274022011. View Article : Google Scholar : PubMed/NCBI | |
Miller MC, Doyle GV and Terstappen LW: Significance of circulating tumor cells detected by the CellSearch system in patients with metastatic breast colorectal and prostate cancer. J Oncol. 2010:6174212010. View Article : Google Scholar | |
Farace F, Massard C, Vimond N, Drusch F, Jacques N, Billiot F, Laplanche A, Chauchereau A, Lacroix L, Planchard D, et al: A direct comparison of CellSearch and ISET for circulating tumour-cell detection in patients with metastatic carcinomas. Br J Cancer. 105:847–853. 2011. View Article : Google Scholar : PubMed/NCBI | |
Alix-Panabières C: EPISPOT assay: Detection of viable DTCs/CTCs in solid tumor patients. Recent Results Cancer Res. 195:69–76. 2012. View Article : Google Scholar : PubMed/NCBI | |
Alix-Panabières C, Vendrell JP, Pellé O, Rebillard X, Riethdorf S, Müller V, Fabbro M and Pantel K: Detection and characterization of putative metastatic precursor cells in cancer patients. Clin Chem. 53:537–539. 2007. View Article : Google Scholar : PubMed/NCBI | |
Hanahan D and Weinberg RA: Hallmarks of cancer: The next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI | |
Somlo G, Lau SK, Frankel P, Hsieh HB, Liu X, Yang L, Krivacic R and Bruce RH: Multiple biomarker expression on circulating tumor cells in comparison to tumor tissues from primary and metastatic sites in patients with locally advanced/inflammatory, and stage IV breast cancer, using a novel detection technology. Breast Cancer Res Treat. 128:155–163. 2011. View Article : Google Scholar : PubMed/NCBI | |
Alix-Panabières C, Vendrell JP, Slijper M, Pellé O, Barbotte E, Mercier G, Jacot W, Fabbro M and Pantel K: Full-length cyto-keratin-19 is released by human tumor cells: A potential role in metastatic progression of breast cancer. Breast Cancer Res. 11:R392009. View Article : Google Scholar | |
Fehm T, Sagalowsky A, Clifford E, Beitsch P, Saboorian H, Euhus D, Meng S, Morrison L, Tucker T, Lane N, et al: Cytogenetic evidence that circulating epithelial cells in patients with carcinoma are malignant. Clin Cancer Res. 8:2073–2084. 2002.PubMed/NCBI | |
Swennenhuis JF, Tibbe AG, Levink R, Sipkema RC and Terstappen LW: Characterization of circulating tumor cells by fluorescence in situ hybridization. Cytometry A. 75:520–527. 2009. View Article : Google Scholar : PubMed/NCBI | |
Bartels CL and Tsongalis GJ: MicroRNAs: Novel biomarkers for human cancer. Clin Chem. 55:623–631. 2009. View Article : Google Scholar : PubMed/NCBI | |
Issadore D, Chung J, Shao H, Liong M, Ghazani AA, Castro CM, Weissleder R and Lee H: Ultrasensitive clinical enumeration of rare cells ex vivo using a micro-hall detector. Sci Transl Med. 4:141ra922012. View Article : Google Scholar : PubMed/NCBI | |
Nagrath S, Sequist LV, Maheswaran S, Bell DW, Irimia D, Ulkus L, Smith MR, Kwak EL, Digumarthy S, Muzikansky A, et al: Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 450:1235–1239. 2007. View Article : Google Scholar : PubMed/NCBI | |
Stott SL, Hsu CH, Tsukrov DI, Yu M, Miyamoto DT, Waltman BA, Rothenberg SM, Shah AM, Smas ME, Korir GK, et al: Isolation of circulating tumor cells using a microvortex-generating herringbone-chip. Proc Natl Acad Sci USA. 107:18392–18397. 2010. View Article : Google Scholar : PubMed/NCBI | |
Sun YY, Lu M, Xi XW, Qiao QQ, Chen LL, Xu XM and Feng YJ: Regulation of epithelial-mesenchymal transition by homeobox gene DLX4 in JEG-3 trophoblast cells: A role in preeclampsia. Reprod Sci. 18:1138–1145. 2011. View Article : Google Scholar : PubMed/NCBI | |
Wang X, Qian X, Beitler JJ, Chen ZG, Khuri FR, Lewis MM, Shin HJ, Nie S and Shin DM: Detection of circulating tumor cells in human peripheral blood using surface-enhanced Raman scattering nanoparticles. Cancer Res. 71:1526–1532. 2011. View Article : Google Scholar : PubMed/NCBI | |
Deng G, Herrler M, Burgess D, Manna E, Krag D and Burke JF: Enrichment with anti-cytokeratin alone or combined with anti-EpCAM antibodies significantly increases the sensitivity for circulating tumor cell detection in metastatic breast cancer patients. Breast Cancer Res. 10:R692008. View Article : Google Scholar : PubMed/NCBI | |
Wang L, Wang Y, Liu Y, Cheng M, Wu X and Wei H: Flow cytometric analysis of CK19 expression in the peripheral blood of breast carcinoma patients: Relevance for circulating tumor cell detection. J Exp Clin Cancer Res. 28:572009. View Article : Google Scholar : PubMed/NCBI | |
Bustin SA and Mueller R: Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis. Clin Sci (Lond). 109:365–379. 2005. View Article : Google Scholar | |
Dubois V, Delort L, Mishellany F, Jarde T, Billard H, Lequeux C, Damour O, Penault-Llorca F, Vasson MP and Caldefie-Chezet F: Zinc-alpha2-glycoprotein: A new biomarker of breast cancer? Anticancer Res. 30:2919–2925. 2010.PubMed/NCBI | |
Fabisiewicz A, Kulik J, Kober P, Brewczyńska E, Pieńkowski T and Siedlecki JA: Detection of circulating breast cancer cells in peripheral blood by a two-marker reverse transcriptase-polymerase chain reaction assay. Acta Biochim Pol. 51:747–755. 2004.PubMed/NCBI | |
Ring A, Smith IE and Dowsett M: Circulating tumour cells in breast cancer. Lancet Oncol. 5:79–88. 2004. View Article : Google Scholar : PubMed/NCBI | |
Van der Auwera I, Peeters D, Benoy IH, Elst HJ, Van Laere SJ, Prové A, Maes H, Huget P, van Dam P, Vermeulen PB, et al: Circulating tumour cell detection: A direct comparison between the CellSearch System, the AdnaTest and CK-19/mammaglobin RT-PCR in patients with metastatic breast cancer. Br J Cancer. 102:276–284. 2010. View Article : Google Scholar : | |
Gibson UE, Heid CA and Williams PM: A novel method for real time quantitative RT-PCR. Genome Res. 6:995–1001. 1996. View Article : Google Scholar : PubMed/NCBI | |
Bustin SA: Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol. 25:169–193. 2000. View Article : Google Scholar : PubMed/NCBI | |
Ginzinger DG: Gene quantification using real-time quantitative PCR: An emerging technology hits the mainstream. Exp Hematol. 30:503–512. 2002. View Article : Google Scholar : PubMed/NCBI | |
Wittwer CT, Herrmann MG, Moss AA and Rasmussen RP: Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques. 22:130–131. 134–138. 1997.PubMed/NCBI | |
Ke LD, Chen Z and Yung WK: A reliability test of standard-based quantitative PCR: Exogenous vs endogenous standards. Mol Cell Probes. 14:127–135. 2000. View Article : Google Scholar : PubMed/NCBI | |
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A and Speleman F: Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3:Research00342002. View Article : Google Scholar : PubMed/NCBI | |
Pfaffl MW, Lange IG, Daxenberger A and Meyer HH: Tissue-specific expression pattern of estrogen receptors (ER): Quantification of ER alpha and ER beta mRNA with real-time RT-PCR. APMIS. 109:345–355. 2001. View Article : Google Scholar : PubMed/NCBI | |
Hocquette JF and Brandstetter AM: Common practice in molecular biology may introduce statistical bias and misleading biological interpretation. J Nutr Biochem. 13:370–377. 2002. View Article : Google Scholar : PubMed/NCBI | |
Ririe KM, Rasmussen RP and Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 245:154–160. 1997. View Article : Google Scholar : PubMed/NCBI | |
Elnifro EM, Ashshi AM, Cooper RJ and Klapper PE: Multiplex PCR: Optimization and application in diagnostic virology. Clin Microbiol Rev. 13:559–570. 2000. View Article : Google Scholar : PubMed/NCBI | |
Max N, Wolf K, Thiel E and Keilholz U: Quantitative nested real-time RT-PCR specific for tyrosinase transcripts to quantitate minimal residual disease. Clin Chim Acta. 317:39–46. 2002. View Article : Google Scholar : PubMed/NCBI | |
Halford WP: The essential prerequisites for quantitative RT-PCR. Nat Biotechnol. 17:8351999. View Article : Google Scholar : PubMed/NCBI | |
Orlando C, Pinzani P and Pazzagli M: Developments in quantitative PCR. Clin Chem Lab Med. 36:255–269. 1998. View Article : Google Scholar : PubMed/NCBI | |
Ghossein RA, Carusone L and Bhattacharya S: Molecular detection of micrometastases and circulating tumor cells in melanoma prostatic and breast carcinomas. In Vivo. 14:237–250. 2000.PubMed/NCBI | |
Cone RW, Hobson AC and Huang ML: Coamplified positive control detects inhibition of polymerase chain reactions. J Clin Microbiol. 30:3185–3189. 1992.PubMed/NCBI | |
Gerges N, Rak J and Jabado N: New technologies for the detection of circulating tumour cells. Br Med Bull. 94:49–64. 2010. View Article : Google Scholar : PubMed/NCBI | |
Chen TF, Jiang GL, Fu XL, Wang LJ, Qian H, Wu KL and Zhao S: CK19 mRNA expression measured by reverse-transcription polymerase chain reaction (RT-PCR) in the peripheral blood of patients with non-small cell lung cancer treated by chemoradiation: An independent prognostic factor. Lung Cancer. 56:105–114. 2007. View Article : Google Scholar | |
Lambrechts AC, Bosma AJ, Klaver SG, Top B, Perebolte L, van' t Veer LJ and Rodenhuis S: Comparison of immunocytochemistry, reverse transcriptase polymerase chain reaction, and nucleic acid sequence-based amplification for the detection of circulating breast cancer cells. Breast Cancer Res Treat. 56:219–231. 1999. View Article : Google Scholar : PubMed/NCBI | |
Lianidou ES and Markou A: Circulating tumor cells in breast cancer: Detection systems, molecular characterization, and future challenges. Clin Chem. 57:1242–1255. 2011. View Article : Google Scholar : PubMed/NCBI | |
Pantel K, Brakenhoff RH and Brandt B: Detection, clinical relevance and specific biological properties of disseminating tumour cells. Nat Rev Cancer. 8:329–340. 2008. View Article : Google Scholar : PubMed/NCBI | |
Gabert J, Beillard E, van der Velden VH, Bi W, Grimwade D, Pallisgaard N, Barbany G, Cazzaniga G, Cayuela JM, Cavé H, et al: Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program. Leukemia. 17:2318–2357. 2003. View Article : Google Scholar : PubMed/NCBI | |
Müller V, Witzel I, Lück HJ, Köhler G, von Minckwitz G, Möbus V, Sattler D, Wilczak W, Löning T, Jänicke F, et al: Prognostic and predictive impact of the HER-2/neu extracellular domain (ECD) in the serum of patients treated with chemotherapy for metastatic breast cancer. Breast Cancer Res Treat. 86:9–18. 2004. View Article : Google Scholar | |
Niesters HG: Standardization and quality control in molecular diagnostics. Expert Rev Mol Diagn. 1:129–131. 2001. View Article : Google Scholar | |
Niesters HG and Puchhammer-Stöckl E: Standardisation and controls, why can't we overcome the hurdles? J Clin Virol. 31:81–83. 2004. View Article : Google Scholar : PubMed/NCBI | |
Raengsakulrach B, Nisalak A, Maneekarn N, Yenchitsomanus PT, Limsomwong C, Jairungsri A, Thirawuth V, Green S, Kalayanarooj S, Suntayakorn S, et al: Comparison of four reverse transcription-polymerase chain reaction procedures for the detection of dengue virus in clinical specimens. J Virol Methods. 105:219–232. 2002. View Article : Google Scholar : PubMed/NCBI | |
Mostert B, Sleijfer S, Foekens JA and Gratama JW: Circulating tumor cells (CTCs): Detection methods and their clinical relevance in breast cancer. Cancer Treat Rev. 35:463–474. 2009. View Article : Google Scholar : PubMed/NCBI | |
Paterlini-Brechot P and Benali NL: Circulating tumor cells (CTC) detection: Clinical impact and future directions. Cancer Lett. 253:180–204. 2007. View Article : Google Scholar : PubMed/NCBI | |
Aktas B, Tewes M, Fehm T, Hauch S, Kimmig R and Kasimir-Bauer S: Stem cell and epithelial-mesenchymal transition markers are frequently overexpressed in circulating tumor cells of metastatic breast cancer patients. Breast Cancer Res. 11:R462009. View Article : Google Scholar : PubMed/NCBI | |
Ignatiadis M, Kallergi G, Ntoulia M, Perraki M, Apostolaki S, Kafousi M, Chlouverakis G, Stathopoulos E, Lianidou E, Georgoulias V, et al: Prognostic value of the molecular detection of circulating tumor cells using a multimarker reverse transcription-PCR assay for cytokeratin 19, mammaglobin A, and HER2 in early breast cancer. Clin Cancer Res. 14:2593–2600. 2008. View Article : Google Scholar : PubMed/NCBI | |
Kowalewska M, Chechlińska M, Markowicz S, Kober P and Nowak R: The relevance of RT-PCR detection of disseminated tumour cells is hampered by the expression of markers regarded as tumour-specific in activated lymphocytes. Eur J Cancer. 42:2671–2674. 2006. View Article : Google Scholar : PubMed/NCBI | |
Suchy B, Austrup F, Driesel G, Eder C, Kusiak I, Uciechowski P, Grill HJ and Giesing M: Detection of mammaglobin expressing cells in blood of breast cancer patients. Cancer Lett. 158:171–178. 2000. View Article : Google Scholar : PubMed/NCBI | |
Jiang Y, Harlocker SL, Molesh DA, Dillon DC, Stolk JA, Houghton RL, Repasky EA, Badaro R, Reed SG and Xu J: Discovery of differentially expressed genes in human breast cancer using subtracted cDNA libraries and cDNA microarrays. Oncogene. 21:2270–2282. 2002. View Article : Google Scholar : PubMed/NCBI | |
Ignatiadis M, Perraki M, Apostolaki S, Politaki E, Xenidis N, Kafousi M, Stathopoulos E, Lianidou E, Sotiriou C, Georgoulias V, et al: Molecular detection and prognostic value of circulating cytokeratin-19 messenger RNA-positive and HER2 messenger RNA-positive cells in the peripheral blood of women with early-stage breast cancer. Clin Breast Cancer. 7:883–889. 2007. View Article : Google Scholar | |
Slade MJ, Smith BM, Sinnett HD, Cross NC and Coombes RC: Quantitative polymerase chain reaction for the detection of micrometastases in patients with breast cancer. J Clin Oncol. 17:870–879. 1999.PubMed/NCBI | |
Stathopoulou A, Mavroudis D, Perraki M, Apostolaki S, Vlachonikolis I, Lianidou E and Georgoulias V: Molecular detection of cancer cells in the peripheral blood of patients with breast cancer: Comparison of CK-19, CEA and maspin as detection markers. Anticancer Res. 23(2C): 1883–1890. 2003.PubMed/NCBI | |
Stathopoulou A, Ntoulia M, Perraki M, Apostolaki S, Mavroudis D, Malamos N, Georgoulias V and Lianidou ES: A highly specific real-time RT-PCR method for the quantitative determination of CK-19 mRNA positive cells in peripheral blood of patients with operable breast cancer. Int J Cancer. 119:1654–1659. 2006. View Article : Google Scholar : PubMed/NCBI | |
Silva HA, Abraúl E, Raimundo D, Dias MF, Marques C, Guerra C, de Oliveira CF and Regateiro FJ: Molecular detection of EGFRvIII-positive cells in the peripheral blood of breast cancer patients. Eur J Cancer. 42:2617–2622. 2006. View Article : Google Scholar : PubMed/NCBI | |
Gargano G, Agnese V, Calò V, Corsale S, Augello C, Bruno L, La Paglia L, Gullo A, Ottini L, Russo A, et al Gruppo Oncologico dell'Italia Meridionale: Detection and quantification of mammaglobin in the blood of breast cancer patients: Can it be useful as a potential clinical marker? Preliminary results of a GOIM (Gruppo Oncologico dell'Italia Meridionale) prospective study. Ann Oncol. 17(Suppl 7): vii41–vii45. 2006. View Article : Google Scholar : PubMed/NCBI | |
Mercatali L, Valenti V, Calistri D, Calpona S, Rosti G, Folli S, Gaudio M, Frassineti GL, Amadori D and Flamini E: RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients. Ann Oncol. 17:424–428. 2006. View Article : Google Scholar | |
Ntoulia M, Stathopoulou A, Ignatiadis M, Malamos N, Mavroudis D, Georgoulias V and Lianidou ES: Detection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer with nested RT-PCR. Clin Biochem. 39:879–887. 2006. View Article : Google Scholar : PubMed/NCBI | |
Fantl V, Smith R, Brookes S, Dickson C and Peters G: Chromosome 11q13 abnormalities in human breast cancer. Cancer Surv. 18:77–94. 1993.PubMed/NCBI | |
Fleming TP and Watson MA: Mammaglobin, a breast-specific gene, and its utility as a marker for breast cancer. Ann NY Acad Sci. 923:78–89. 2000. View Article : Google Scholar | |
Ceballos MP, Zumoffen C, Massa E, Cipulli G, Funes CC, Gil AB, Morales C, Tozzini R and Ghersevich S: Detection of mammaglogin A in blood from breast cancer patients, before and after treatment, using a one-tube nested PCR protocol. Association with the absence of tumor estrogen receptors. Clin Biochem. 44:1429–1433. 2011. View Article : Google Scholar : PubMed/NCBI | |
Corradini P, Voena C, Astolfi M, Delloro S, Pilotti S, Arrigoni G, Bregni M, Pileri A and Gianni AM: Maspin and mammaglobin genes are specific markers for RT-PCR detection of minimal residual disease in patients with breast cancer. Ann Oncol. 12:1693–1698. 2001. View Article : Google Scholar | |
Ferro P, Franceschini MC, Bacigalupo B, Dessanti P, Falco E, Fontana V, Gianquinto D, Pistillo MP, Fedeli F and Roncella S: Detection of circulating tumour cells in breast cancer patients using human mammaglobin RT-PCR: Association with clinical prognostic factors. Anticancer Res. 30:2377–2382. 2010.PubMed/NCBI | |
Roncella S, Ferro P, Bacigalupo B, Pronzato P, Tognoni A, Falco E, Gianquinto D, Ansaldo V, Dessanti P, Fais F, et al: Human mammaglobin mRNA is a reliable molecular marker for detecting occult breast cancer cells in peripheral blood. J Exp Clin Cancer Res. 24:265–271. 2005.PubMed/NCBI | |
Silva AL, Tomé MJ, Correia AE and Passos-Coelho JL: Human mammaglobin RT-PCR assay for detection of occult breast cancer cells in hematopoietic products. Ann Oncol. 13:422–429. 2002. View Article : Google Scholar : PubMed/NCBI | |
Ballestrero A, Garuti A, Bertolotto M, Rocco I, Boy D, Nencioni A, Ottonello L and Patrone F: Effect of different cytokines on mammaglobin and maspin gene expression in normal leukocytes: Possible relevance to the assays for the detection of micrometastatic breast cancer. Br J Cancer. 92:1948–1952. 2005. View Article : Google Scholar : PubMed/NCBI | |
Cerveira N, Torres L, Rocha P, Bizarro S, Pereira D, Abreu J, Henrique R, Teixeira MR and Castedo S: Highly sensitive detection of the MGB1 transcript (mammaglobin) in the peripheral blood of breast cancer patients. Int J Cancer. 108:592–595. 2004. View Article : Google Scholar | |
Zach O, Kasparu H, Krieger O, Hehenwarter W, Girschikofsky M and Lutz D: Detection of circulating mammary carcinoma cells in the peripheral blood of breast cancer patients via a nested reverse transcriptase polymerase chain reaction assay for mammaglobin mRNA. J Clin Oncol. 17:2015–2019. 1999.PubMed/NCBI | |
Silva JM, Domínguez G, González-Sancho JM, García JM, Silva J, García-Andrade C, Navarro A, Muñoz A and Bonilla F: Expression of thyroid hormone receptor/erbA genes is altered in human breast cancer. Oncogene. 21:4307–4316. 2002. View Article : Google Scholar : PubMed/NCBI | |
Tewes M, Aktas B, Welt A, Mueller S, Hauch S, Kimmig R and Kasimir-Bauer S: Molecular profiling and predictive value of circulating tumor cells in patients with metastatic breast cancer: An option for monitoring response to breast cancer related therapies. Breast Cancer Res Treat. 115:581–590. 2009. View Article : Google Scholar | |
Shen C, Hu L, Xia L and Li Y: The detection of circulating tumor cells of breast cancer patients by using multimarker (Survivin, hTERT and hMAM) quantitative real-time PCR. Clin Biochem. 42:194–200. 2009. View Article : Google Scholar | |
Zehentner BK, Persing DH, Deme A, Toure P, Hawes SE, Brooks L, Feng Q, Hayes DC, Critichlow CW, Houghton RL, et al: Mammaglobin as a novel breast cancer biomarker: Multigene reverse transcription-PCR assay and sandwich ELISA. Clin Chem. 50:2069–2076. 2004. View Article : Google Scholar : PubMed/NCBI | |
Barrallo-Gimeno A and Nieto MA: The Snail genes as inducers of cell movement and survival: Implications in development and cancer. Development. 132:3151–3161. 2005. View Article : Google Scholar : PubMed/NCBI | |
Monteiro J and Fodde R: Cancer stemness and metastasis: Therapeutic consequences and perspectives. Eur J Cancer. 46:1198–1203. 2010. View Article : Google Scholar : PubMed/NCBI | |
Sabbah M, Emami S, Redeuilh G, Julien S, Prévost G, Zimber A, Ouelaa R, Bracke M, De Wever O and Gespach C: Molecular signature and therapeutic perspective of the epithelial-to-mesenchymal transitions in epithelial cancers. Drug Resist Updat. 11:123–151. 2008. View Article : Google Scholar : PubMed/NCBI | |
Thompson R: Cancer: Epithelial subtype influences the prognosis of invasive IPMN. Nat Rev Gastroenterol Hepatol. 8:4202011. View Article : Google Scholar : PubMed/NCBI | |
Gradilone A, Raimondi C, Nicolazzo C, Petracca A, Gandini O, Vincenzi B, Naso G, Aglianò AM, Cortesi E and Gazzaniga P: Circulating tumour cells lacking cytokeratin in breast cancer: The importance of being mesenchymal. J Cell Mol Med. 15:1066–1070. 2011. View Article : Google Scholar : PubMed/NCBI | |
Bednarz-Knoll N, Alix-Panabières C and Pantel K: Plasticity of disseminating cancer cells in patients with epithelial malignancies. Cancer Metastasis Rev. 31:673–687. 2012. View Article : Google Scholar : PubMed/NCBI | |
Smirnov DA, Zweitzig DR, Foulk BW, Miller MC, Doyle GV, Pienta KJ, Meropol NJ, Weiner LM, Cohen SJ, Moreno JG, et al: Global gene expression profiling of circulating tumor cells. Cancer Res. 65:4993–4997. 2005. View Article : Google Scholar : PubMed/NCBI | |
de Albuquerque A, Kaul S, Breier G, Krabisch P and Fersis N: Multimarker analysis of circulating tumor cells in peripheral blood of metastatic breast cancer patients: A step forward in personalized medicine. Breast Care (Basel). 7:7–12. 2012. View Article : Google Scholar | |
Lowes LE and Allan AL: Recent advances in the molecular characterization of circulating tumor cells. Cancers (Basel). 6:595–624. 2014. View Article : Google Scholar | |
Grover PK, Cummins AG, Price TJ, Roberts-Thomson IC and Hardingham JE: Circulating tumour cells: The evolving concept and the inadequacy of their enrichment by EpCAM-based methodology for basic and clinical cancer research. Ann Oncol. 25:1506–1516. 2014. View Article : Google Scholar : PubMed/NCBI | |
Liu X and Fan D: The epithelial-mesenchymal transition and cancer stem cells: Functional and mechanistic links. Curr Pharm Des. 21:1279–1291. 2014. View Article : Google Scholar : PubMed/NCBI | |
Čabiňaková M and Tesařová P: Disseminated and circulating tumour cells and their role in breast cancer. Folia Biol (Praha). 58:87–97. 2012. | |
Daskalaki A, Agelaki S, Perraki M, Apostolaki S, Xenidis N, Stathopoulos E, Kontopodis E, Hatzidaki D, Mavroudis D and Georgoulias V: Detection of cytokeratin-19 mRNA-positive cells in the peripheral blood and bone marrow of patients with operable breast cancer. Br J Cancer. 101:589–597. 2009. View Article : Google Scholar : PubMed/NCBI | |
Andergassen U, Hofmann S, Kölbl AC, Schindlbeck C, Neugebauer J, Hutter S, Engelstädter V, Ilmer M, Friese K and Jeschke U: Detection of tumor cell-specific mRNA in the peripheral blood of patients with breast cancer-evaluation of several markers with real-time reverse transcription-PCR. Int J Mol Sci. 14:1093–1104. 2013. View Article : Google Scholar : PubMed/NCBI | |
Cierna Z, Mego M, Janega P, Karaba M, Minarik G, Benca J, Sedlácková T, Cingelova S, Gronesova P, Manasova D, et al: Matrix metalloproteinase 1 and circulating tumor cells in early breast cancer. BMC Cancer. 14:4722014. View Article : Google Scholar : PubMed/NCBI | |
Cheng M, Chen Y, Zou D, Shen G, Bian G, Shen G and Hu S: The clinical utility of circulating tumor cells in breast cancer patients: Detection by a quantitative assay of h-MAM gene expression. Int J Biol Markers. 29:e268–e278. 2014. View Article : Google Scholar : PubMed/NCBI | |
Xi L, Nicastri DG, El-Hefnawy T, Hughes SJ, Luketich JD and Godfrey TE: Optimal markers for real-time quantitative reverse transcription PCR detection of circulating tumor cells from melanoma, breast, colon, esophageal, head and neck, and lung cancers. Clin Chem. 53:1206–1215. 2007. View Article : Google Scholar : PubMed/NCBI | |
Lasa A, Garcia A, Alonso C, Millet P, Cornet M, Ramón Y, Cajal T, Baiget M and Barnadas A: Molecular detection of peripheral blood breast cancer mRNA transcripts as a surrogate biomarker for circulating tumor cells. PLoS One. 8:e740792013. View Article : Google Scholar : PubMed/NCBI | |
Skondra M, Gkioka E, Kostakis ID, Pissimissis N, Lembessis P, Pectasides D and Koutsilieris M: Detection of circulating tumor cells in breast cancer patients using multiplex reverse transcription-polymerase chain reaction and specific primers for MGB, PTHRP and KRT19 correlation with clinicopathological features. Anticancer Res. 34:6691–6699. 2014.PubMed/NCBI | |
Zebisch M, Kölbl AC, Schindlbeck C, Neugebauer J, Heublein S, Ilmer M, Rack B, Friese K, Jeschke U and Andergassen U: Quantification of breast cancer cells in peripheral blood samples by real-time RT-PCR. Anticancer Res. 32:5387–5391. 2012.PubMed/NCBI | |
Guo M, Li X, Zhang S, Song H, Zhang W, Shang X, Zheng Y, Jiang H, Lv Q, Jiang Y, et al: Real-time quantitative RT-PCR detection of circulating tumor cells from breast cancer patients. Int J Oncol. 46:281–289. 2015. | |
Pinzani P, Salvadori B, Simi L, Bianchi S, Distante V, Cataliotti L, Pazzagli M and Orlando C: Isolation by size of epithelial tumor cells in peripheral blood of patients with breast cancer: Correlation with real-time reverse transcriptase-polymerase chain reaction results and feasibility of molecular analysis by laser microdissection. Hum Pathol. 37:711–718. 2006. View Article : Google Scholar : PubMed/NCBI | |
Nakagawa T, Martinez SR, Goto Y, Koyanagi K, Kitago M, Shingai T, Elashoff DA, Ye X, Singer FR, Giuliano AE, et al: Detection of circulating tumor cells in early-stage breast cancer metastasis to axillary lymph nodes. Clin Cancer Res. 13:4105–4110. 2007. View Article : Google Scholar : PubMed/NCBI | |
Zhang L, Ridgway LD, Wetzel MD, Ngo J, Yin W, Kumar D, Goodman JC, Groves MD and Marchetti D: The identification and characterization of breast cancer CTCs competent for brain metastasis. Sci Transl Med. 5:180ra482013. View Article : Google Scholar : PubMed/NCBI | |
Androulakis N, Agelaki S, Perraki M, Apostolaki S, Bozionelou V, Pallis A, Kalbakis K, Xyrafas A, Mavroudis D and Georgoulias V: Clinical relevance of circulating CK-19mRNA-positive tumour cells before front-line treatment in patients with metastatic breast cancer. Br J Cancer. 106:1917–1925. 2012. View Article : Google Scholar : PubMed/NCBI | |
Georgoulias V, Apostolaki S, Bozionelou V, Politaki E, Perraki M, Georgoulia N, Kalbakis K, Kotsakis A, Xyrafas A, Agelaki S, et al: Effect of front-line chemotherapy on circulating CK-19 mRNA-positive cells in patients with metastatic breast cancer. Cancer Chemother Pharmacol. 74:1217–1225. 2014. View Article : Google Scholar : PubMed/NCBI | |
Xenidis N, Perraki M, Apostolaki S, Agelaki S, Kalbakis K, Vardakis N, Kalykaki A, Xyrafas A, Kakolyris S, Mavroudis D, et al: Differential effect of adjuvant taxane-based and taxane-free chemotherapy regimens on the CK-19 mRNA-positive circulating tumour cells in patients with early breast cancer. Br J Cancer. 108:549–556. 2013. View Article : Google Scholar : PubMed/NCBI | |
Wang HY, Ahn S, Kim S, Park S, Park S, Han H, Sohn JH, Kim S and Lee H: Detection of circulating tumor cells in patients with breast cancer using the quantitative RT-PCR assay for monitoring of therapy efficacy. Exp Mol Pathol. 97:445–452. 2014. View Article : Google Scholar : PubMed/NCBI | |
Ušiaková Z, Mikulová V, Pintérová D, Brychta M, Valchář J, Kubecová M, Tesařová P, Bobek V and Kološtová K: Circulating tumor cells in patients with breast cancer: Monitoring chemotherapy success. In Vivo. 28:605–614. 2014.PubMed/NCBI | |
Mikulová V, Cabiňaková M, Janatková I, Mestek O, Zima T and Tesařová P: Detection of circulating tumor cells during follow-up of patients with early breast cancer: Clinical utility for monitoring of therapy efficacy. Scand J Clin Lab Invest. 74:132–142. 2014. View Article : Google Scholar | |
Mego M, Mani SA, Lee BN, Li C, Evans KW, Cohen EN, Gao H, Jackson SA, Giordano A, Hortobagyi GN, et al: Expression of epithelial-mesenchymal transition-inducing transcription factors in primary breast cancer: The effect of neoadjuvant therapy. Int J Cancer. 130:808–816. 2012. View Article : Google Scholar | |
Baccelli I, Stenzinger A, Vogel V, Pfitzner BM, Klein C, Wallwiener M, Scharpff M, Saini M, Holland-Letz T, Sinn HP, et al: Co-expression of MET and CD47 is a novel prognosticator for survival of luminal breast cancer patients. Oncotarget. 5:8147–8160. 2014. View Article : Google Scholar : PubMed/NCBI | |
Sterzynska K, Kempisty B, Zawierucha P and Zabel M: Analysis of the specificity and selectivity of anti-EpCAM antibodies in breast cancer cell lines. Folia Histochem Cytobiol. 50:534–541. 2012. View Article : Google Scholar : PubMed/NCBI | |
Markiewicz A, Książkiewicz M, Seroczyńska B, Skokowski J, Szade J, Wełnicka-Jaśkiewicz M and Zaczek AJ: Heterogeneity of mesenchymal markers expression-molecular profiles of cancer cells disseminated by lymphatic and hematogenous routes in breast cancer. Cancers (Basel). 5:1485–1503. 2013. View Article : Google Scholar | |
Serrano MJ, Ortega FG, Alvarez-Cubero MJ, Nadal R, Sanchez-Rovira P, Salido M, Rodríguez M, García-Puche JL, Delgado-Rodriguez M, Solé F, et al: EMT and EGFR in CTCs cytokeratin negative non-metastatic breast cancer. Oncotarget. 5:7486–7497. 2014. View Article : Google Scholar : PubMed/NCBI | |
Sieuwerts AM, Mostert B, Bolt-de Vries J, Peeters D, de Jongh FE, Stouthard JM, Dirix LY, van Dam PA, Van Galen A, de Weerd V, et al: mRNA and microRNA expression profiles in circulating tumor cells and primary tumors of metastatic breast cancer patients. Clin Cancer Res. 17:3600–3618. 2011. View Article : Google Scholar : PubMed/NCBI | |
Shaw JA, Brown J, Coombes RC, Jacob J, Payne R, Lee B, Page K, Hava N and Stebbing J: Circulating tumor cells and plasma DNA analysis in patients with indeterminate early or metastatic breast cancer. Biomarkers Med. 5:87–91. 2011. View Article : Google Scholar | |
Peeters DJ, Brouwer A, Van den Eynden GG, Rutten A, Onstenk W, Sieuwerts AM, Van Laere SJ, Huget P, Pauwels P, Peeters M, et al: Circulating tumour cells and lung microvascular tumour cell retention in patients with metastatic breast and cervical cancer. Cancer Lett. 356(2 Pt B): 872–879. 2015. View Article : Google Scholar | |
Kurata H, Takakuwa K, Tsuneki I, Aoki Y and Tanaka K: Ovarian tumor cell detection in peripheral blood progenitor cells harvests by RT-PCR. Acta Obstet Gynecol Scand. 81:555–559. 2002. View Article : Google Scholar : PubMed/NCBI | |
Oikonomopoulou K, Scorilas A, Michael IP, Grass L, Soosaipillai A, Rosen B, Murphy J and Diamandis EP: Kallikreins as markers of disseminated tumour cells in ovarian cancer: a pilot study. Tumour Biol. 27:104–114. 2006. View Article : Google Scholar | |
Magnowski P, Bochyński H, Nowak-Markwitz E, Zabel M and Spaczyński M: Circulating tumor cells (CTCs)–clinical significance in patients with ovarian cancer. Ginekol Pol. 83:291–294. 2012.In Polish. PubMed/NCBI | |
Poveda A, Kaye SB, McCormack R, Wang S, Parekh T, Ricci D, Lebedinsky CA, Tercero JC, Zintl P and Monk BJ: Circulating tumor cells predict progression free survival and overall survival in patients with relapsed/recurrent advanced ovarian cancer. Gynecol Oncol. 122:567–572. 2011. View Article : Google Scholar : PubMed/NCBI | |
Romero-Laorden N, Olmos D, Fehm T, Garcia-Donas J and Diaz-Padilla I: Circulating and disseminated tumor cells in ovarian cancer: A systematic review. Gynecol Oncol. 133:632–639. 2014. View Article : Google Scholar : PubMed/NCBI | |
Liu JF, Kindelberger D, Doyle C, Lowe A, Barry WT and Matulonis UA: Predictive value of circulating tumor cells (CTCs) in newly-diagnosed and recurrent ovarian cancer patients. Gynecol Oncol. 131:352–356. 2013. View Article : Google Scholar : PubMed/NCBI | |
Aktas B, Kasimir-Bauer S, Heubner M, Kimmig R and Wimberger P: Molecular profiling and prognostic relevance of circulating tumor cells in the blood of ovarian cancer patients at primary diagnosis and after platinum-based chemotherapy. Int J Gynecol Cancer. 21:822–830. 2011. View Article : Google Scholar : PubMed/NCBI | |
Obermayr E, Castillo-Tong DC, Pils D, Speiser P, Braicu I, Van Gorp T, Mahner S, Sehouli J, Vergote I and Zeillinger R: Molecular characterization of circulating tumor cells in patients with ovarian cancer improves their prognostic significance - a study of the OVCAD consortium. Gynecol Oncol. 128:15–21. 2013. View Article : Google Scholar | |
Pearl ML, Zhao Q, Yang J, Dong H, Tulley S, Zhang Q, Golightly M, Zucker S and Chen WT: Prognostic analysis of invasive circulating tumor cells (iCTCs) in epithelial ovarian cancer. Gynecol Oncol. 134:581–590. 2014. View Article : Google Scholar : PubMed/NCBI | |
Zeng L, Liang X, Liu Q and Yang Z: The predictive value of circulating tumor cells in ovarian cancer: A meta analysis. Int J Gynecol Cancer. Apr 17–2015.Epub ahead of print. | |
Song J and Nettles JB: Circulating tumor cells in patients with carcinoma in situ of the cervix uteri. Am J Obstet Gynecol. 104:713–726. 1969.PubMed/NCBI | |
Yuan CC, Wang PH, Ng HT, Li YF, Huang TS, Chen CY, Tsai LC and Shyong WY: Detecting cytokeratin 19 mRNA in the peripheral blood cells of cervical cancer patients and its clinical-pathological correlation. Gynecol Oncol. 85:148–153. 2002. View Article : Google Scholar : PubMed/NCBI | |
Weismann P, Weismanova E, Masak L, Mlada K, Keder D, Ferancikova Z, Vizvaryova M, Konecny M, Zavodna K, Kausitz J, et al: The detection of circulating tumor cells expressing E6/E7 HR-HPV oncogenes in peripheral blood in cervical cancer patients after radical hysterectomy. Neoplasma. 56:230–238. 2009. View Article : Google Scholar : PubMed/NCBI | |
Alonso-Alconada L, Muinelo-Romay L, Madissoo K, Diaz-Lopez A, Krakstad C, Trovik J, Wik E, Hapangama D, Coenegrachts L, Cano A, et al ENITEC Consortium: Molecular profiling of circulating tumor cells links plasticity to the metastatic process in endometrial cancer. Mol Cancer. 13:2232014. View Article : Google Scholar : PubMed/NCBI | |
Bogani G, Liu MC, Dowdy SC, Cliby WA, Kerr SE, Kalli KR, Kipp BR, Halling KC, Campion MB and Mariani A: Detection of circulating tumor cells in high-risk endometrial cancer. Anticancer Res. 35:683–687. 2015.PubMed/NCBI | |
Grünewald K, Haun M, Fiegl M, Urbanek M, Müller-Holzner E, Massoner A, Riha K, Propst A, Marth C and Gastl G: Mammaglobin expression in gynecologic malignancies and malignant effusions detected by nested reverse transcriptase-polymerase chain reaction. Lab Invest. 82:1147–1153. 2002. View Article : Google Scholar : PubMed/NCBI | |
Obermayr E, Sanchez-Cabo F, Tea MK, Singer CF, Krainer M, Fischer MB, Sehouli J, Reinthaller A, Horvat R, Heinze G, et al: Assessment of a six gene panel for the molecular detection of circulating tumor cells in the blood of female cancer patients. BMC Cancer. 10:6662010. View Article : Google Scholar : PubMed/NCBI |