Assessment of sFRP4 as a bio-marker for predicting aggressiveness and recurrence of growth hormone-secreting pituitary adenomas

  • Authors:
    • Youtu Wu
    • Chunhui Liu
    • Shengyuan Yu
    • Hua Gao
    • Zhenye Li
    • Chuzhong Li
    • Yazhuo Zhang
  • View Affiliations

  • Published online on: March 3, 2016     https://doi.org/10.3892/or.2016.4650
  • Pages: 2991-2999
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Abstract

The association of sFRP4 expression with aggressiveness and recurrence of growth hormone (GH)-secreting pituitary adenomas was investigated. Ten normal pituitary and 52 GH-secreting pituitary adenoma specimens were classified into three groups: normal pituitary (control) group, non-aggressive group, and aggressive group, according to preoperative evaluation by magnetic resonance imaging (MRI)/computed tomography (CT). Expression of sFRP4 was determined by quantitative real-time polymerase chain reaction (qRT-PCR), western blot analysis, and tissue microarrays, to assess the association between sFRP4 and aggressiveness. Follow-up information of all 52 patients was collected to evaluate the impact of sFRP4 expression on the recurrence/progression of GH-secreting pituitary adenomas. qRT-PCR results showed a lower level of sFRP4 mRNA in the aggressive group, as compared to that in the non-aggressive group (P=0.001). A similar trend was observed on western blot analysis for sFRP4 protein expression (P=0.004). On analysis by tissue microarrays, weak sFRP4 expression was detected in the aggressive group (10/15, 66.7%). Univariate analysis showed a significant relationship between low sFRP4 expression and aggressiveness (P=0.024). On multivariate analysis weak sFRP4 expression was found to be an independent factor of recurrence/progression (odds ratio: 0.063, P=0.026). Methylation of the sFRP4 promoter was increased in low sFRP4 staining group compared to that in the high sFRP4 staining group (P<0.001). In this study, weak sFRP4 expression appeared to predict aggressive behavior, and was associated with recurrence/progression of GH-secreting pituitary adenomas. Methylation of the sFRP4 promoter may account for the low sFRP4 expression.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wu Y, Liu C, Yu S, Gao H, Li Z, Li C and Zhang Y: Assessment of sFRP4 as a bio-marker for predicting aggressiveness and recurrence of growth hormone-secreting pituitary adenomas. Oncol Rep 35: 2991-2999, 2016.
APA
Wu, Y., Liu, C., Yu, S., Gao, H., Li, Z., Li, C., & Zhang, Y. (2016). Assessment of sFRP4 as a bio-marker for predicting aggressiveness and recurrence of growth hormone-secreting pituitary adenomas. Oncology Reports, 35, 2991-2999. https://doi.org/10.3892/or.2016.4650
MLA
Wu, Y., Liu, C., Yu, S., Gao, H., Li, Z., Li, C., Zhang, Y."Assessment of sFRP4 as a bio-marker for predicting aggressiveness and recurrence of growth hormone-secreting pituitary adenomas". Oncology Reports 35.5 (2016): 2991-2999.
Chicago
Wu, Y., Liu, C., Yu, S., Gao, H., Li, Z., Li, C., Zhang, Y."Assessment of sFRP4 as a bio-marker for predicting aggressiveness and recurrence of growth hormone-secreting pituitary adenomas". Oncology Reports 35, no. 5 (2016): 2991-2999. https://doi.org/10.3892/or.2016.4650