MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells

  • Authors:
    • Enrica Fabbri
    • Giulia Montagner
    • Nicoletta Bianchi
    • Alessia Finotti
    • Monica Borgatti
    • Ilaria Lampronti
    • Giulio Cabrini
    • Roberto Gambari
  • View Affiliations

  • Published online on: March 15, 2016     https://doi.org/10.3892/or.2016.4676
  • Pages: 2866-2872
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Abstract

The role of the microRNA miR-93-5p on the secretome profile and the expression levels of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) was investigated in the neuroblastoma SK-N-AS cell line by Bio-Plex analysis and RT-qPCR. The results indicate that VEGF and IL-8 are the major miR-93-5p molecular targets. This conclusion was based on in vitro transfection with pre-miR-93-5p and anti-miR-93-5p; these treatments inversely modulated both VEGF and IL-8 gene expression and protein release in the neuroblastoma SK-N-AS cell line. Computational analysis showed the presence of miR-93-5p consensus sequences in the 3'UTR region of both VEGF and IL-8 mRNAs, predicting possible interaction with miR-93-5p and confirming a potential regulatory role of this microRNA.
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May-2016
Volume 35 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fabbri E, Montagner G, Bianchi N, Finotti A, Borgatti M, Lampronti I, Cabrini G and Gambari R: MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells. Oncol Rep 35: 2866-2872, 2016.
APA
Fabbri, E., Montagner, G., Bianchi, N., Finotti, A., Borgatti, M., Lampronti, I. ... Gambari, R. (2016). MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells. Oncology Reports, 35, 2866-2872. https://doi.org/10.3892/or.2016.4676
MLA
Fabbri, E., Montagner, G., Bianchi, N., Finotti, A., Borgatti, M., Lampronti, I., Cabrini, G., Gambari, R."MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells". Oncology Reports 35.5 (2016): 2866-2872.
Chicago
Fabbri, E., Montagner, G., Bianchi, N., Finotti, A., Borgatti, M., Lampronti, I., Cabrini, G., Gambari, R."MicroRNA miR-93-5p regulates expression of IL-8 and VEGF in neuroblastoma SK-N-AS cells". Oncology Reports 35, no. 5 (2016): 2866-2872. https://doi.org/10.3892/or.2016.4676