Stromal interaction molecule 1 plays an important role in gastric cancer progression

Xu,Jin-Mei; Zhou,Yan; Gao,Long; Zhou,Shu-Xian; Liu,Wei-Hua; Li,Xiao-An

doi:10.3892/or.2016.4704

Stromal interaction molecule 1 plays an important role in gastric cancer progression

Authors:
- Jin-Mei Xu
- Yan Zhou
- Long Gao
- Shu-Xian Zhou
- Wei-Hua Liu
- Xiao-An Li
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Affiliations: The Gastroenterology Tumor and Microenvironment Laboratory, Department of Gastroenterology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, Xindu, Chengdu, Sichuan 610041, P.R. China
Published online on: March 24, 2016 https://doi.org/10.3892/or.2016.4704
Pages: 3496-3504

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Abstract

Studies have shown that stromal interaction molecule 1 (STIM1) is expressed in a variety of cancers and is related to tumor growth. The present study aimed to investigate the expression and roles of STIM1 in gastric carcinoma. Immunohistochemistry and western blotting revealed that STIM1 was expressed at higher levels in gastric cancer tissues (82%) than these levels in normal gastric tissues (42%). In addition, STIM1 was also expressed in tumor vascular endothelial cells. The effects of STIM1 on proliferation, apoptosis, adhesion, invasion and migration of gastric cancer cells were detected by MTT assay, flow cytometry, cell adhesion assay and Transwell assay, respectively. The results shown that STIM1 knockdown did not alter proliferation or apoptosis, but promoted cell adhesion and inhibited migration and invasion in the gastric cancer cells. In addition, STIM1 knockdown did not alter the expression or phosphorylation of mitogen-activated protein kinase (MEK) or extracellular signal-regulated kinase (ERK), implying that STIM1 affected gastric cancer cell migration through a pathway independent of the MEK/ERK pathway.

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1	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar
2	Berridge MJ, Bootman MD and Roderick HL: Calcium signalling: Dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol. 4:517–529. 2003. View Article : Google Scholar : PubMed/NCBI
3	Zheng L, Stathopulos PB, Li GY and Ikura M: Biophysical characterization of the EF-hand and SAM domain containing Ca²⁺ sensory region of STIM1 and STIM2. Biochem Biophys Res Commun. 369:240–246. 2008. View Article : Google Scholar : PubMed/NCBI
4	Oh-Hora M, Yamashita M, Hogan PG, Sharma S, Lamperti E, Chung W, Prakriya M, Feske S and Rao A: Dual functions for the endoplasmic reticulum calcium sensors STIM1 and STIM2 in T cell activation and tolerance. Nat Immunol. 9:432–443. 2008. View Article : Google Scholar : PubMed/NCBI
5	Hewavitharana T, Deng X, Soboloff J and Gill DL: Role of STIM and Orai proteins in the store-operated calcium signaling pathway. Cell Calcium. 42:173–182. 2007. View Article : Google Scholar : PubMed/NCBI
6	Muik M, Schindl R, Fahrner M and Romanin C: Ca²⁺ release-activated Ca²⁺ (CRAC) current, structure, and function. Cell Mol Life Sci. 69:4163–4176. 2012. View Article : Google Scholar : PubMed/NCBI
7	Stathopulos PB, Zheng L, Li GY, Plevin MJ and Ikura M: Structural and mechanistic insights into STIM1-mediated initiation of store-operated calcium entry. Cell. 135:110–122. 2008. View Article : Google Scholar : PubMed/NCBI
8	Omilusik KD, Nohara LL, Stanwood S and Jefferies WA: Weft, warp, and weave: The intricate tapestry of calcium channels regulating T lymphocyte function. Front Immunol. 4:1642013. View Article : Google Scholar : PubMed/NCBI
9	Matsumoto M and Baba Y: Role of STIM-dependent Ca²⁺ influx in regulatory B cells. Yakugaku Zasshi. 133:419–425. 2013.In Japanese. View Article : Google Scholar
10	Félix R, Crottès D, Delalande A, Fauconnier J, Lebranchu Y, Le Guennec JY and Velge-Roussel F: The Orai-1 and STIM-1 complex controls human dendritic cell maturation. PLoS One. 8:e615952013. View Article : Google Scholar : PubMed/NCBI
11	Lee KJ, Woo JS, Hwang JH, Hyun C, Cho CH, Kim do H and Lee EH: STIM1 negatively regulates Ca²⁺ release from the sarcoplasmic reticulum in skeletal myotubes. Biochem J. 453:187–200. 2013. View Article : Google Scholar : PubMed/NCBI
12	Fodor J, Matta C, Oláh T, Juhász T, Takács R, Tóth A, Dienes B, Csernoch L and Zákány R: Store-operated calcium entry and calcium influx via voltage-operated calcium channels regulate intracellular calcium oscillations in chondrogenic cells. Cell Calcium. 54:1–16. 2013. View Article : Google Scholar : PubMed/NCBI
13	Yang S, Zhang JJ and Huang XY: Orai1 and STIM1 are critical for breast tumor cell migration and metastasis. Cancer Cell. 15:124–134. 2009. View Article : Google Scholar : PubMed/NCBI
14	Chen YF, Chiu WT, Chen YT, Lin PY, Huang HJ, Chou CY, Chang HC, Tang MJ and Shen MR: Calcium store sensor stromal-interaction molecule 1-dependent signaling plays an important role in cervical cancer growth, migration, and angiogenesis. Proc Natl Acad Sci USA. 108:15225–15230. 2011. View Article : Google Scholar : PubMed/NCBI
15	Yang N, Tang Y, Wang F, Zhang H, Xu D, Shen Y, Sun S and Yang G: Blockade of store-operated Ca²⁺ entry inhibits hepatocarcinoma cell migration and invasion by regulating focal adhesion turnover. Cancer Lett. 330:163–169. 2013. View Article : Google Scholar
16	Sun S, Li W, Zhang H, Zha L, Xue Y, Wu X and Zou F: Requirement for store-operated calcium entry in sodium butyrate-induced apoptosis in human colon cancer cells. Biosci Rep. 32:83–90. 2012. View Article : Google Scholar
17	Suyama E, Wadhwa R, Kaur K, Miyagishi M, Kaul SC, Kawasaki H and Taira K: Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes. J Biol Chem. 279:38083–38086. 2004. View Article : Google Scholar : PubMed/NCBI
18	Abdullaev IF, Bisaillon JM, Potier M, Gonzalez JC, Motiani RK and Trebak M: Stim1 and Orai1 mediate CRAC currents and store-operated calcium entry important for endothelial cell proliferation. Circ Res. 103:1289–1299. 2008. View Article : Google Scholar : PubMed/NCBI
19	Lodola F, Laforenza U, Bonetti E, Lim D, Dragoni S, Bottino C, Ong HL, Guerra G, Ganini C, Massa M, et al: Store-operated Ca²⁺ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients. PLoS One. 7:e425412012. View Article : Google Scholar
20	Sabbioni S, Barbanti-Brodano G, Croce CM and Negrini M: GOK: A gene at 11p15 involved in rhabdomyosarcoma and rhabdoid tumor development. Cancer Res. 57:4493–4497. 1997.PubMed/NCBI
21	Li W, Zhang M, Xu L, Lin D, Cai S and Zou F: The apoptosis of non-small cell lung cancer induced by cisplatin through modulation of STIM1. Exp Toxicol Pathol. 65:1073–1081. 2013. View Article : Google Scholar : PubMed/NCBI
22	Cerne JZ, Stegel V, Gersak K, Novakovic S and Jasmina-Ziva: KRAS rs61764370 is associated with HER2-overexpressed and poorly-differentiated breast cancer in hormone replacement therapy users: A case control study. BMC Cancer. 12:1052012. View Article : Google Scholar : PubMed/NCBI
23	Sui JQ, Xie KP, Zou W and Xie MJ: Emodin inhibits breast cancer cell proliferation through the ERα-MAPK/Akt-cyclin D1/Bcl-2 signaling pathway. Asian Pac J Cancer Prev. 15:6247–6251. 2014. View Article : Google Scholar
24	Zhong LR, Chen X and Wei KM: Radix tetrastigma hemsleyani flavone induces apoptosis in human lung carcinoma a549 cells by modulating the MAPK pathway. Asian Pac J Cancer Prev. 14:5983–5987. 2013. View Article : Google Scholar : PubMed/NCBI
25	de Mello RA, Marques DS, Medeiros R and Araújo AM: Epidermal growth factor receptor and K-Ras in non-small cell lung cancer-molecular pathways involved and targeted therapies. World J Clin Oncol. 2:367–376. 2011. View Article : Google Scholar : PubMed/NCBI
26	Ono H, Basson MD and Ito H: PTK6 promotes cancer migration and invasion in pancreatic cancer cells dependent on ERK signaling. PLoS One. 9:e960602014. View Article : Google Scholar : PubMed/NCBI
27	Neuzillet C, Hammel P, Tijeras-Raballand A, Couvelard A and Raymond E: Targeting the Ras-ERK pathway in pancreatic adenocarcinoma. Cancer Metastasis Rev. 32:147–162. 2013. View Article : Google Scholar
28	Nagini S: Carcinoma of the stomach: A review of epidemiology, pathogenesis, molecular genetics and chemoprevention. World J Gastrointest Oncol. 4:156–169. 2012. View Article : Google Scholar : PubMed/NCBI