HOTTIP and HOXA13 are oncogenes associated with gastric cancer progression

  • Authors:
    • Shuai Chang
    • Junsong Liu
    • Shaochun Guo
    • Shicai He
    • Guanglin Qiu
    • Jing Lu
    • Jin Wang
    • Lin Fan
    • Wei Zhao
    • Xiangming Che
  • View Affiliations

  • Published online on: April 13, 2016     https://doi.org/10.3892/or.2016.4743
  • Pages: 3577-3585
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Abstract

A long non-coding RNA named HOTTIP (HOXA transcript at the distal tip) coordinates the activation of various 5' HOXA genes which encode master regulators of development through targeting the WDR5/MLL complex. HOTTIP acts as an oncogene in several types of cancers, whereas its biological function in gastric cancer has never been studied. In the present study, we investigated the role of HOTTIP in gastric cancer. We found that HOTTIP was upregulated in gastric cancer cell lines. Knockdown of HOTTIP in gastric cancer cells inhibited cell proliferation, migration and invasion. Moreover, downregulation of HOTTIP led to decreased expression of homeobox protein Hox-A13 (HOXA13) in gastric cancer cell lines. HOXA13 was involved in HOTTIP‑induced malignant phenotypes of gastric cancer cells. Our data showed that the levels of HOTTIP and HOXA13 were both markedly upregulated in gastric cancer tissues compared with their counterparts in non-tumorous tissues. Furthermore, the expression levels of HOTTIP and HOXA13 were both higher in gastric cancer which was poorly differentiated, at advanced TNM stages and exhibited lymph node-metastasis. Spearman analyses indicated that HOTTIP and HOXA13 had a highly positive correlation both in non-tumor mucosae and cancer lesions. Collectively, these findings suggest that HOTTIP and HOXA13 play important roles in gastric cancer progression and provide a new insight into therapeutic treatment for the disease.
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June-2016
Volume 35 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chang S, Liu J, Guo S, He S, Qiu G, Lu J, Wang J, Fan L, Zhao W, Che X, Che X, et al: HOTTIP and HOXA13 are oncogenes associated with gastric cancer progression. Oncol Rep 35: 3577-3585, 2016.
APA
Chang, S., Liu, J., Guo, S., He, S., Qiu, G., Lu, J. ... Che, X. (2016). HOTTIP and HOXA13 are oncogenes associated with gastric cancer progression. Oncology Reports, 35, 3577-3585. https://doi.org/10.3892/or.2016.4743
MLA
Chang, S., Liu, J., Guo, S., He, S., Qiu, G., Lu, J., Wang, J., Fan, L., Zhao, W., Che, X."HOTTIP and HOXA13 are oncogenes associated with gastric cancer progression". Oncology Reports 35.6 (2016): 3577-3585.
Chicago
Chang, S., Liu, J., Guo, S., He, S., Qiu, G., Lu, J., Wang, J., Fan, L., Zhao, W., Che, X."HOTTIP and HOXA13 are oncogenes associated with gastric cancer progression". Oncology Reports 35, no. 6 (2016): 3577-3585. https://doi.org/10.3892/or.2016.4743