Open Access

STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer

  • Authors:
    • Yao Cui
    • Yun-Yun Li
    • Jian Li
    • Hong-Yan Zhang
    • Feng Wang
    • Xue Bai
    • Shan-Shan Li
  • View Affiliations

  • Published online on: May 19, 2016     https://doi.org/10.3892/or.2016.4822
  • Pages: 108-116
  • Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hypoxia plays a key role in tumour initiation and metastasis; one of the mechanisms is to induce epithelial-mesenchymal transition (EMT). Signal transducer and activator of transcription 3 (STAT3) is involved in EMT by regulating the transcriptional regulators of E-cadherin, the biomarker of EMT. Until now, however, few studies have focused on the effects of STAT3 in hypoxia-induced EMT in tumour cells. The goal of this study was to investigate the roles of STAT3 in hypoxia-induced EMT in oesophageal squamous cell carcinoma (ESCC). The ESCC cells, TE-1 and EC-1, were incubated in normoxia, or in CoCl2, which was used to mimic hypoxia. With CoCl2, the ESCC cells showed increased migration and invasion abilities, accompanied with upregulation of HIF-1α, STAT3, and vimentin, and downregulation of E-cadherin. Knockdown of STAT3 inhibited EMT of ESCC cells and downregulated HIF-1α in vitro and in vivo. In ChIP assays, STAT3 bound to the promoter of HIF-1α, suggesting that STAT3 regulates transcription of HIF-1α. In conclusion, hypoxia induces EMT of ESCC, and STAT3 regulates this process by promoting HIF-1α expression.
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July-2016
Volume 36 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Cui Y, Li Y, Li J, Zhang H, Wang F, Bai X and Li S: STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer. Oncol Rep 36: 108-116, 2016.
APA
Cui, Y., Li, Y., Li, J., Zhang, H., Wang, F., Bai, X., & Li, S. (2016). STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer. Oncology Reports, 36, 108-116. https://doi.org/10.3892/or.2016.4822
MLA
Cui, Y., Li, Y., Li, J., Zhang, H., Wang, F., Bai, X., Li, S."STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer". Oncology Reports 36.1 (2016): 108-116.
Chicago
Cui, Y., Li, Y., Li, J., Zhang, H., Wang, F., Bai, X., Li, S."STAT3 regulates hypoxia-induced epithelial mesenchymal transition in oesophageal squamous cell cancer". Oncology Reports 36, no. 1 (2016): 108-116. https://doi.org/10.3892/or.2016.4822