Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer

  • Authors:
    • Mengxuan Yang
    • Yingying Qu
    • Rongliang Shi
    • Xubo Wu
    • Chang Su
    • Zhiqiu Hu
    • Qimeng Chang
    • Shaoqun Liu
    • Gaofeng Pan
    • Ming Lei
    • Fubo Xie
    • Shiwei Tu
    • Weikang Tao
    • He Zhou
    • Gang Hu
    • Ziping Zhang
  • View Affiliations

  • Published online on: June 23, 2016     https://doi.org/10.3892/or.2016.4906
  • Pages: 779-786
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Abstract

Gastric cancer is a fatal disease and the availability of early diagnostic methods is limited. There is an urgent need to identify effective targets for early diagnosis and therapeutics. UbcH10 is a ubiquitin-conjugating enzyme with high expression in various types of cancers. In the present study, several gastric tumor cell lines with high or low expression of UbcH10 were exploited to study the role of UbcH10 in gastric cancer. Knockdown of UbcH10 expression using siRNA in gastric cancer cell lines with high expression of UbcH10 resulted in reduced proliferation, increased cisplatin-induced apoptosis and reduced serum-induced ERK, Akt and p38 phosphorylation signaling. In agreement, overexpression of UbcH10 in gastric cancer cell lines with low expression of UbcH10 led to enhanced cell proliferation and resistance to cisplatin-induced apoptosis. Most importantly, IHC analyses showed that the UbcH10 protein was expressed at a high level in most patient gastric cancer tissues, but was absent in adjacent mesenchyme tissues. These data suggest that UbcH10 may promote gastric cancer growth and can serve as a biomarker for diagnosis or as a target for novel therapeutics in gastric cancer.
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August-2016
Volume 36 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yang M, Qu Y, Shi R, Wu X, Su C, Hu Z, Chang Q, Liu S, Pan G, Lei M, Lei M, et al: Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer. Oncol Rep 36: 779-786, 2016.
APA
Yang, M., Qu, Y., Shi, R., Wu, X., Su, C., Hu, Z. ... Zhang, Z. (2016). Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer. Oncology Reports, 36, 779-786. https://doi.org/10.3892/or.2016.4906
MLA
Yang, M., Qu, Y., Shi, R., Wu, X., Su, C., Hu, Z., Chang, Q., Liu, S., Pan, G., Lei, M., Xie, F., Tu, S., Tao, W., Zhou, H., Hu, G., Zhang, Z."Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer". Oncology Reports 36.2 (2016): 779-786.
Chicago
Yang, M., Qu, Y., Shi, R., Wu, X., Su, C., Hu, Z., Chang, Q., Liu, S., Pan, G., Lei, M., Xie, F., Tu, S., Tao, W., Zhou, H., Hu, G., Zhang, Z."Ubiquitin-conjugating enzyme UbcH10 promotes gastric cancer growth and is a potential biomarker for gastric cancer". Oncology Reports 36, no. 2 (2016): 779-786. https://doi.org/10.3892/or.2016.4906