miR-15a induces cell apoptosis by targeting BCL2L2 and BCL2 in HPV-positive hypopharyngeal squamous cell carcinoma

Lu,Wuhao; Feng,Long; Zhang,Yan; Ma,Yunyun; Li,Ping; Wang,Yuanyuan; Du,Yuwen; Chen,Xiaonan; Wu,Shujun; Zhao,Guoqiang; Lou,Weihua

doi:10.3892/or.2016.5049

miR-15a induces cell apoptosis by targeting BCL2L2 and BCL2 in HPV-positive hypopharyngeal squamous cell carcinoma

Authors:
- Wuhao Lu
- Long Feng
- Yan Zhang
- Yunyun Ma
- Ping Li
- Yuanyuan Wang
- Yuwen Du
- Xiaonan Chen
- Shujun Wu
- Guoqiang Zhao
- Weihua Lou
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Affiliations: Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pathogenic Organism Biology, Henan University of Chinese Medicine, Zhengzhou, Henan 450008, P.R. China, Department of Immunology and Microbiology, Henan Medical College, Zhengzhou, Henan 451191, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
Published online on: August 25, 2016 https://doi.org/10.3892/or.2016.5049
Pages: 2169-2176

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Abstract

Aberrant expression of miR-15a was recently reported in several types of cancers; however, its role in HPV-positive hypopharyngeal squamous cell carcinoma (HSCC) remains obscure. In the present study, we investigated the mechanism by which miR-15a induces HPV-positive HSCC apoptosis. Synthetic miR-15a mimics were transfected into FaDu cells (HPV-negative), and the miR-15a inhibitor was transfected into HPV-positive HSCC cells. miR-15a expression was analyzed by RT-PCR, and BCL2L2 and BCL2 were analyzed by western blotting. The Hochest 33342/propidium iodide (PI) and caspase-3/-9 assays, and Annexin V staining were used to assess the effect of miR-15a on apoptosis. After transfection, overexpression of miR-15a in the FaDu cells was associated with significantly decreased BCL2L2 and BCL2 expression and a significant increase in the apoptosis rate. The opposite results were observed in HPV-positive HSCC, where downregulation of miR-15a suppressed apoptosis. These findings indicate that miR-15a acts as a tumor suppressor in HPV-positive HSCC.

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