13-Methyl-palmatrubine induces apoptosis and cell cycle arrest in A549 cells in vitro and in vivo

Chen,Jingxian; Lu,Xingang; Lu,Chenghua; Wang,Chunying; Xu,Haizhu; Xu,Xiaoli; Gou,Haixin; Zhu,Bing; Du,Wangchun

doi:10.3892/or.2016.5093

13-Methyl-palmatrubine induces apoptosis and cell cycle arrest in A549 cells in vitro and in vivo

Authors:
- Jingxian Chen
- Xingang Lu
- Chenghua Lu
- Chunying Wang
- Haizhu Xu
- Xiaoli Xu
- Haixin Gou
- Bing Zhu
- Wangchun Du
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Affiliations: Department of Traditional Chinese Medicine, RuiJin Hospital, JiaoTong University School of Medicine, Shanghai 200025, P.R. China, Department of Traditional Chinese Medicine, HuaDong Hospital, FuDan University School of Medicine, Shanghai 200040, P.R. China, Department of Respiration, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China, Department of Oncology, LongHua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, P.R. China, Clinical Medicine College, Shanghai University of Medicine and Health Sciences, Shanghai 201318, P.R. China
Published online on: September 15, 2016 https://doi.org/10.3892/or.2016.5093
Pages: 2526-2534
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Corydalis yanhusuo, a well-known herbaceous plant, is commonly used in the treatment of inflammation, injury and pain. One natural agent isolated from Corydalis yanhusuo, 13-methyl-palmatrubine, was found to have a cytotoxic effect on cancer cells as reported in published studies. In the present study, we synthesized a potential anti-lung tumor agent, 13-methyl-palmatrubine and analyzed its activity. 13-Methyl-palmatrubine exhibited a cytotoxic effect on a panel of cancer cell lines in a time- and concentration-dependent manner. Among all the tested cancer cell lines, lung cancer A549 cells were most sensitive to 13-methyl-palmatrubine treatment. Meanwhile 13-methyl-palmatrubine showed less cytotoxicity in human normal cells. Our investigation revealed that 13-methyl‑palmatrubine induced apoptosis and cell cycle arrest in A549 cells in a dose-dependent manner. Furthermore, 13-methyl-palmatrubine treatment caused activation of P38 and JNK pathways and blocked the EGFR pathway. In conclusion, our findings demonstrated that 13-methyl-palmatrubine inhibited the growth of A549 cells mediated by blocking of the EGFR signaling pathway and activation of the MAPK signaling pathway and provides a better understanding of the molecular mechanisms of 13-methyl-palmatrubine.

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