Autophagy eliminates ER membrane reorganization induced by Bcl-2 inhibitor in HeLa cells

Li,Xiaoning; Kang,Yongwei; Jiao,Bingxuan; Xia,Meihui; Wu,Yao; Sun,Liankun

doi:10.3892/or.2016.5209

Autophagy eliminates ER membrane reorganization induced by Bcl-2 inhibitor in HeLa cells

Authors:
- Xiaoning Li
- Yongwei Kang
- Bingxuan Jiao
- Meihui Xia
- Yao Wu
- Liankun Sun
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Affiliations: Department of Pathophysiology, Basic Medical College, Jilin University, Changchun, Jilin 130021, P.R. China, School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. China, Department of Obstetrics and Gynecology, The First Hospital, Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: October 27, 2016 https://doi.org/10.3892/or.2016.5209
Pages: 3353-3362

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Abstract

The endoplasmic reticulum (ER) is a membranous network within cells that is important for several cellular functions including translation and folding of secretory and membrane proteins, lipid biogenesis and sequestration of Ca2+. Disruption of ER structure might affect the normal physiology of the cells. In yeast, expansion of the ER is observed under unfolded protein response (UPR) and subsequently induces autophagy initiated from the ER. In this study, we demonstrated a drastic and specific ER membrane reorganization (EMR), characterized by the clustering of the ER membrane into large and compact aggregates and occurring independent of UPR in HeLa cells treated with S1 combined with ABT-737. Subsequently, combined with S1 and ABT-737 triggered autophagy. Herein, we report a key step for removal of damaged and superfluous cellular constituents, by a mechanistic link between ER aggregation and autophagic activation. Our study is the first time to show that autophagy may be a way to remove the ER membrane reorganization induced by Bcl-2 inhibitors ABT-737 and S1 and it may help us to analyze autophagy in certain diseases.

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