Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma

  • Authors:
    • Yanting Liu
    • Zhaowei Chu
    • Qingyan Li
    • Bin Peng
    • Suyun Xu
    • Christine G. Lian
    • Songmei Geng
  • View Affiliations

  • Published online on: November 15, 2016     https://doi.org/10.3892/or.2016.5244
  • Pages: 139-146
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Abstract

Epithelial-mesenchymal transition (EMT) contributes to the invasion and metastasis of numerous malignant cancers, including melanoma. A significant higher expression of B-lymphoma Moloney murine leukemia virus insertion region-1 (Bmi-1) has been reported in cell lines from metastatic melanoma compared to cell lines from primary melanoma. There are studies that show that knockdown of Bmi-1 could induce E-cadherin expression in melanoma cells. However, the role of Bmi-1 in mediating EMT-like changes in melanoma has not yet been fully studied. In the present study, knockdown of Bmi-1 by shRNA transduction decreased the invasion properties of the cultured human melanoma cells A375 by a Matrigel invasion assay, along with alterations in EMT-related markers E-cadherin, α-catenin, vimentin and N-cadherin. The aforementioned altered expression of EMT markers was verified in BALB/c-nude mouse xenografts. Furthermore, to explore the underlying regulatory mechanism of EMT, we detected the significant downregulation of p-Akt/p‑NF-κB/MMP-2 and the upregulation of PTEN in Bmi-1-silenced A375 cells. The present study demonstrated that knockdown of Bmi-1 significantly inhibited the aggressive behavior of melanoma by reversing EMT-like changes via the PTEN/p-Akt/p‑NF-κB/MMP-2 pathway.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Liu Y, Chu Z, Li Q, Peng B, Xu S, Lian CG and Geng S: Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma. Oncol Rep 37: 139-146, 2017.
APA
Liu, Y., Chu, Z., Li, Q., Peng, B., Xu, S., Lian, C.G., & Geng, S. (2017). Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma. Oncology Reports, 37, 139-146. https://doi.org/10.3892/or.2016.5244
MLA
Liu, Y., Chu, Z., Li, Q., Peng, B., Xu, S., Lian, C. G., Geng, S."Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma". Oncology Reports 37.1 (2017): 139-146.
Chicago
Liu, Y., Chu, Z., Li, Q., Peng, B., Xu, S., Lian, C. G., Geng, S."Downregulation of Bmi-1 suppresses epithelial‑mesenchymal transition in melanoma". Oncology Reports 37, no. 1 (2017): 139-146. https://doi.org/10.3892/or.2016.5244