HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling

  • Authors:
    • Shudong Gu
    • Rui Zhang
    • Jun Gu
    • Xia Li
    • Liting Lv
    • Jingting Jiang
    • Zhen Xu
    • Shuo Wang
    • Cui Shi
    • Dan Ping Wang
    • Changping Wu
  • View Affiliations

  • Published online on: November 22, 2016     https://doi.org/10.3892/or.2016.5268
  • Pages: 474-482
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

HES5 is a transcription factor activated downstream of the Notch pathway and regulates cell differentiation in multiple tissues. Disruption of its normal expression has been associated with developmental diseases and cancer. But its role in non-small cell lung cancer (NSCLC) remains elusive. Western blot analysis and immunohistochemistry assays demonstrated that HES5 expression was frequently increased in NSCLC tumor tissues and cell lines. Moreover, immunohistochemistry analysis revealed that upregulation of HES5 expression was positively correlated with a more invasive tumor phenotype and poor prognosis. Immunoprecipitation assay indicated that HES5 directly interacted with STAT3. In addition, depletion of HES5 resulted in inhibited phosphorylation of STAT3 and decreased expression of the downstream genes. In vitro studies, using serum starvation-refeeding experiment and HES5-siRNA transfection assay demonstrated that HES5 expression promoted proliferation of NSCLC cells, while HES5 knockdown caused growth arrest of cell cycle at G0/G1 phase, decreased rate of colony formation and alleviated cellular apoptosis. Taken together, out data have delineated that HES5 might contribute to the proliferation of NSCLC by STAT3 signaling.
View Figures
View References

Related Articles

Journal Cover

January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gu S, Zhang R, Gu J, Li X, Lv L, Jiang J, Xu Z, Wang S, Shi C, Wang DP, Wang DP, et al: HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling. Oncol Rep 37: 474-482, 2017.
APA
Gu, S., Zhang, R., Gu, J., Li, X., Lv, L., Jiang, J. ... Wu, C. (2017). HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling. Oncology Reports, 37, 474-482. https://doi.org/10.3892/or.2016.5268
MLA
Gu, S., Zhang, R., Gu, J., Li, X., Lv, L., Jiang, J., Xu, Z., Wang, S., Shi, C., Wang, D. P., Wu, C."HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling". Oncology Reports 37.1 (2017): 474-482.
Chicago
Gu, S., Zhang, R., Gu, J., Li, X., Lv, L., Jiang, J., Xu, Z., Wang, S., Shi, C., Wang, D. P., Wu, C."HES5 promotes cellular proliferation of non-small cell lung cancer through STAT3 signaling". Oncology Reports 37, no. 1 (2017): 474-482. https://doi.org/10.3892/or.2016.5268