miRNA-301a induces apoptosis of chronic myelogenous leukemia cells by directly targeting TIMP2/ERK1/2 and AKT pathways

  • Authors:
    • Xianbao Huang
    • Ling Qi
    • Wei Lu
    • Gangping Yang
    • Yan Chen
    • Rongyan Zhang
    • Jia Rao
    • Dexiang Ji
    • Ruibin Huang
    • Guoan Chen
  • View Affiliations

  • Published online on: December 22, 2016     https://doi.org/10.3892/or.2016.5330
  • Pages: 945-952
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Abstract

We investigated the biological functions and mechanism of miRNA‑301a on apoptosis in chronic myelogenous leukemia (CML). The expression of miRNA‑301a in patient with CML cells was higher than the expression of normal patients. Overall survival (OS) of chronic granulocytic leukemia cell patient with low miRNA‑301 expression was superior to that of CML patient with high miRNA‑301 expression. Moreover, the upregulation of miRNA‑301a increased cell proliferation, inhibited apoptosis and caspase-3 and -9 activity of K562 cells. Next, the upregulation of miRNA‑301a suppressed Bax/Bcl-2 rate and TIMP2 protein expression, increased phosphorylation-ERK1/2 and decreased phosphorylation-AKT protein expression of K562 cells. Furthermore, si‑TIMP2 expression enhanced the upregulation of miRNA‑301a on the promotion of cell proliferation, inhibition of apoptosis and caspase-3 and -9 activity, suppression of Bax/Bcl-2 rate, increasing phosphorylation-ERK1/2 and decreasing phosphorylation-AKT protein expression of K562 cells. Taken together, our results clearly suggested that miRNA‑301a induces apoptosis of CML cells by directly targeting the TIMP2/ERK1/2 and AKT pathways.
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February-2017
Volume 37 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Huang X, Qi L, Lu W, Yang G, Chen Y, Zhang R, Rao J, Ji D, Huang R, Chen G, Chen G, et al: miRNA-301a induces apoptosis of chronic myelogenous leukemia cells by directly targeting TIMP2/ERK1/2 and AKT pathways. Oncol Rep 37: 945-952, 2017.
APA
Huang, X., Qi, L., Lu, W., Yang, G., Chen, Y., Zhang, R. ... Chen, G. (2017). miRNA-301a induces apoptosis of chronic myelogenous leukemia cells by directly targeting TIMP2/ERK1/2 and AKT pathways. Oncology Reports, 37, 945-952. https://doi.org/10.3892/or.2016.5330
MLA
Huang, X., Qi, L., Lu, W., Yang, G., Chen, Y., Zhang, R., Rao, J., Ji, D., Huang, R., Chen, G."miRNA-301a induces apoptosis of chronic myelogenous leukemia cells by directly targeting TIMP2/ERK1/2 and AKT pathways". Oncology Reports 37.2 (2017): 945-952.
Chicago
Huang, X., Qi, L., Lu, W., Yang, G., Chen, Y., Zhang, R., Rao, J., Ji, D., Huang, R., Chen, G."miRNA-301a induces apoptosis of chronic myelogenous leukemia cells by directly targeting TIMP2/ERK1/2 and AKT pathways". Oncology Reports 37, no. 2 (2017): 945-952. https://doi.org/10.3892/or.2016.5330