Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling

Corrigendum in: /10.3892/or.2024.8734

  • Authors:
    • Yan Liu
    • Zhenbang Qin
    • Kuo Yang
    • Ranlu Liu
    • Yong Xu
  • View Affiliations

  • Published online on: January 17, 2017     https://doi.org/10.3892/or.2017.5378
  • Pages: 1521-1528
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Abstract

The Cripto-1 (CR-1) derived EGF-CFC family was overexpressed in tumor development enhancing proliferation, epithelial-mesenchymal transition (EMT) and migration of tumor cells. However, correlation between CR-1 and prostate cancer (PCa) remains still unclear. In the present study, we proved that CR-1 was expressed in PCa and its function was in the progression of PCa. Compared with benign prostatic hyperplasia (BPH) tissues, we confirmed that PCa tissues had high expression of CR-1 by immunohistochemistry and statistical data showed that CR-1 promoted properties of EMT in PCa tissues, including the downregulation of the cell adhesion molecules β-catenin (membrane) and E-cadherin while upregulating transcription factors β-catenin. Overexpression of CR-1 had close relationship with PSA, Gleason, clinical staging and lymph node metastasis in PCa patients. Then, we found that PC-3 cells transfected with CR-1-shRNA inhibited EMT using RT-PCR, RT-qPCR, western blotting and immunofluorescence. Also, we evaluated cell invasive ability in vitro by transwell and wound-healing assay. Our data showed that transfected CR-1-shRNA altered EMT including β-catenin, E-cadherin, c-myc, GSK-3, p-GSK and Wnt/β-catenin pathway in PC-3. It also suppressed PC-3 cell migration. Additionally, our results displayed that Licl had antitumor activity against PC-3 through the inhibition of Wnt/β-catenin pathway. Inhibition of cell viability was dose-time dependent. The present study proved that CR-1 regulates EMT of PCa by Wnt/β-catenin pathway. Hence, CR-1 may provide a new biological marker, and possibly contributes to clinical treatment against PCa.
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March-2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu Y, Qin Z, Yang K, Liu R and Xu Y: Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncol Rep 37: 1521-1528, 2017.
APA
Liu, Y., Qin, Z., Yang, K., Liu, R., & Xu, Y. (2017). Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734. Oncology Reports, 37, 1521-1528. https://doi.org/10.3892/or.2017.5378
MLA
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37.3 (2017): 1521-1528.
Chicago
Liu, Y., Qin, Z., Yang, K., Liu, R., Xu, Y."Cripto-1 promotes epithelial-mesenchymal transition in prostate cancer via Wnt/β-catenin signaling Corrigendum in /10.3892/or.2024.8734". Oncology Reports 37, no. 3 (2017): 1521-1528. https://doi.org/10.3892/or.2017.5378