High expression of PINK1 promotes proliferation and chemoresistance of NSCLC

Zhang,Rui; Gu,Jun; Chen,Jie; Ni,Jun; Hung,Jieru; Wang,Zhiwen; Zhang,Xiaochen; Feng,Jian; Ji,Lili

doi:10.3892/or.2017.5486

High expression of PINK1 promotes proliferation and chemoresistance of NSCLC

Authors:
- Rui Zhang
- Jun Gu
- Jie Chen
- Jun Ni
- Jieru Hung
- Zhiwen Wang
- Xiaochen Zhang
- Jian Feng
- Lili Ji
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Affiliations: Department of Respiratory Disease, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Oncology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Rehabilitation, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226200, P.R. China, Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, Jiangsu 226001, P.R. China, Department of Intensive Care Unit, Qidong People's Hospital, Nantong, Jiangsu 226200, P.R. China, Department of Pathology, Medical College of Nantong University, Nantong, Jiangsu 226001, P.R. China
Published online on: March 2, 2017 https://doi.org/10.3892/or.2017.5486
Pages: 2137-2146

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Abstract

PTEN-induced putative kinase 1 (PINK1) was identified initially as a gene upregulated in cancer cells which regulates cellular processes of significance in cancer cell biology, including cell survival, stress resistance and the cell cycle. However, the expression and function of PINK1 in non-small cell lung cancer (NSCLC) has not been determined yet. We demonstrated high PINK1 expression in NSCLC tumor tissues and cell lines as assessed by western blot and immunohistochemistry (IHC) assays. In addition, IHC analysis revealed that PINK1 expression was associated with a more invasive tumor phenotype and poor prognosis. Furthermore, in vitro studies using upregulation and knockdown of PINK1 confirmed that PINK1 promoted cell proliferation of NSCLC, which might be through as the NF-κB pathway. Moreover, we also demonstrated that downregulation of PINK1 enhanced cisplatin (CDDP)-induced NSCLC cell apoptosis. Together, our findings indicate that PINK1 plays a significant role in NSCLC progression and chemoresistance, and highlights its potential role as a target in future anticancer therapies.

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