miR-200a targets Gelsolin: A novel mechanism regulating secretion of microvesicles in hepatocellular carcinoma cells

Xu,Ya; Zhang,Yanfen; Wang,Lujing; Zhao,Ruiqi; Qiao,Yu; Han,Dong; Sun,Qian; Dong,Nazhen; Liu,Yicong; Wu,Dantong; Zhang,Xuemei; Huang,Ning; Ma,Ning; Zhao,Weiming; Liu,Yanhong; Gao,Xu

doi:10.3892/or.2017.5506

miR-200a targets Gelsolin: A novel mechanism regulating secretion of microvesicles in hepatocellular carcinoma cells

Authors:
- Ya Xu
- Yanfen Zhang
- Lujing Wang
- Ruiqi Zhao
- Yu Qiao
- Dong Han
- Qian Sun
- Nazhen Dong
- Yicong Liu
- Dantong Wu
- Xuemei Zhang
- Ning Huang
- Ning Ma
- Weiming Zhao
- Yanhong Liu
- Xu Gao
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Affiliations: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, P.R. China, Department of Laboratory Diagnosis, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, P.R. China
Published online on: March 15, 2017 https://doi.org/10.3892/or.2017.5506
Pages: 2711-2719
Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Microvesicle biogenesis is a highly regulated process. Aberrant release of microvesicles from cancer cells have been associated with their invasiveness and prognosis. However, the mechanism of aberrant release remains poorly understood. Herein, we found that hepatocellular carcinoma cells shed more microvesicles than normal hepatocytes and miR-200a were shown to inhibit the release of microvesicles in hepatocellular carcinoma cells. Then, we confirmed that miR-200a might target Gelsolin and change cytoskeleton to regulate microvesicles secretion. Further miR-200a may inhibit the proliferation of adjacent cells by inhibiting the release of microvesicles. Collectively, our findings indicate that miR-200a regulated the microvesicle biogenesis involved in the hepatocellular carcinoma progression.

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