Open Access

Identification and expression of MMSA-8, and its clinical significance in multiple myeloma

  • Authors:
    • Rui He
    • Nan Yang
    • Pengyu Zhang
    • Jie Liu
    • Junhui Li
    • Fulin Zhou
    • Wanggang Zhang
  • View Affiliations

  • Published online on: April 28, 2017     https://doi.org/10.3892/or.2017.5609
  • Pages: 3235-3243
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In our previous studies, we identified 12 multiple myeloma (MM)-associated antigens by serological analysis of tumor-associated antigens with a recombinant cDNA expression library (SEREX) on MM. MM-associated antigen-8 (MMSA-8) was one of the new antigens identified. We determined the 3'- and 5'-ends of MMSA-8 using SMART-rapid amplification of cDNA ends (RACE) and then cloned its full-length cDNA in the U266 cell line. The full cDNA sequence revealed that MMSA-8 is RPS27A-related transcript variant 1 that is specifically associated with MM. We examined its prognostic significance for the first time, by investigating the correlations between MMSA-8 expression and definite clinicopathological features. We quantitatively assessed MMSA-8 expression using qRT-PCR and western blot analysis in healthy donors and MM patients. The expression levels of MMSA-8 were upregulated with statistical significance in MM patients in contrast to those in healthy donors. The expression of MMSA-8 was also upregulated in relapsed patients compared with that in the complete remission (CR) group. Contrasting MMSA-8 expression levels in different patients with definite clinicopathological features suggested an association between MMSA-8 with unfavorable clinicopathological characteristics, such as international staging system (ISS) stage III, higher lactate dehydrogenase (LDH) levels and higher C-reactive protein (CRP) levels. The expression of MMSA-8 was also increased in patients with unfavorable cytogenetic and genetic abnormalities, including the presence of t(11;14), t(4;14), t(14;16), del(17p), del(13q) and p53 deletion, which was statistically significant. The expression of MMSA-8 exhibited significant variance in the treatment responses of the CR, PR, progression and relapse groups. Univariate and multivariate analyses revealed that high MMSA-8 values were associated with poorer progression-free survival (PFS) and overall survival (OS) in MM patients independently. In conclusion, our data indicated that MMSA-8 is an independent and unfavorable prognostic risk factor in MM; MMSA-8 is also a promising diagnostic and therapeutic target in MM patients, but further validation is needed.
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June-2017
Volume 37 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
He R, Yang N, Zhang P, Liu J, Li J, Zhou F and Zhang W: Identification and expression of MMSA-8, and its clinical significance in multiple myeloma. Oncol Rep 37: 3235-3243, 2017.
APA
He, R., Yang, N., Zhang, P., Liu, J., Li, J., Zhou, F., & Zhang, W. (2017). Identification and expression of MMSA-8, and its clinical significance in multiple myeloma. Oncology Reports, 37, 3235-3243. https://doi.org/10.3892/or.2017.5609
MLA
He, R., Yang, N., Zhang, P., Liu, J., Li, J., Zhou, F., Zhang, W."Identification and expression of MMSA-8, and its clinical significance in multiple myeloma". Oncology Reports 37.6 (2017): 3235-3243.
Chicago
He, R., Yang, N., Zhang, P., Liu, J., Li, J., Zhou, F., Zhang, W."Identification and expression of MMSA-8, and its clinical significance in multiple myeloma". Oncology Reports 37, no. 6 (2017): 3235-3243. https://doi.org/10.3892/or.2017.5609