Effect of miR-1244 on cisplatin-treated non-small cell lung cancer via MEF2D expression

  • Authors:
    • Guang-Jian Li
    • Guang-Qiang Zhao
    • Jia-Peng Yang
    • Yong-Chun Zhou
    • Kai-Yun Yang
    • Yu-Jie Lei
    • Yun-Chao Huang
  • View Affiliations

  • Published online on: May 4, 2017     https://doi.org/10.3892/or.2017.5624
  • Pages: 3475-3483
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Abstract

The aim of this study was to investigate the function of miR-1244 in cisplatin-treated non-small cell lung cancer (NSCLC). The results of quantitative PCR analysis revealed that the expression levels of miR-1244 in cisplatin‑treated A549 and NCI-H522 human lung cancer cell lines were lower than those in untreated A549 and NCI-H522 cells. Similarly, the expression level of miR-1244 in NSCLC tissue samples from cisplatin-treated patients was also lower than that in non-cisplatin-treated NSCLC patients. Notably, the overall survival times of cisplatin-treated NSCLC patients with high miR-1244 expression were superior to those patients with low miR-1244 expression. We found that overexpression of miR-1244 suppressed cell viability and increased LDH toxicity in cisplatin-treated A549 and NCI-H522 cells. Additionally, overexpression of miR-1244 induced the apoptosis of cisplatin-treated A549 and NCI-H522 cells. Furthermore, overexpression of miR-1244 promoted caspase-3 activity and p53 and Bax protein expression, and suppressed myocyte enhancer factor 2D (MEF2D) and cyclin D1 protein expression in cisplatin‑treated A549 and NCI-H522 cells. Small interfering RNA (siRNA) targeting MEF2D suppressed the protein expression of MEF2D, and was able to decrease the proliferation, promote caspase-3 activity, p53 and Bax protein expression and inhibit cyclin D1 protein expression in cisplatin-treated A549 and NCI-H522 cells following the overexpression of miR-1244. In summary, we found that miR-1244 affected cisplatin-treated NSCLC via MEF2D expression.
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June-2017
Volume 37 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li G, Zhao G, Yang J, Zhou Y, Yang K, Lei Y and Huang Y: Effect of miR-1244 on cisplatin-treated non-small cell lung cancer via MEF2D expression. Oncol Rep 37: 3475-3483, 2017.
APA
Li, G., Zhao, G., Yang, J., Zhou, Y., Yang, K., Lei, Y., & Huang, Y. (2017). Effect of miR-1244 on cisplatin-treated non-small cell lung cancer via MEF2D expression. Oncology Reports, 37, 3475-3483. https://doi.org/10.3892/or.2017.5624
MLA
Li, G., Zhao, G., Yang, J., Zhou, Y., Yang, K., Lei, Y., Huang, Y."Effect of miR-1244 on cisplatin-treated non-small cell lung cancer via MEF2D expression". Oncology Reports 37.6 (2017): 3475-3483.
Chicago
Li, G., Zhao, G., Yang, J., Zhou, Y., Yang, K., Lei, Y., Huang, Y."Effect of miR-1244 on cisplatin-treated non-small cell lung cancer via MEF2D expression". Oncology Reports 37, no. 6 (2017): 3475-3483. https://doi.org/10.3892/or.2017.5624