Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1

Zhao,Jing; Lv,Kexing; Li,Zeng-Hong; Wu,Jianhui; Gao,Wei; Wong,Thian-Sze; Luo,Jie; Qin,Hao; Wang,Bin; Fu,Qingling; Lei,Wen-Bin

doi:10.3892/or.2017.5645

Functional significance of the long non-coding RNA RP11-169D4.1 as a metastasis suppressor in laryngeal squamous cell carcinoma by regulating CDH1

Authors:
- Jing Zhao
- Kexing Lv
- Zeng-Hong Li
- Jianhui Wu
- Wei Gao
- Thian-Sze Wong
- Jie Luo
- Hao Qin
- Bin Wang
- Qingling Fu
- Wen-Bin Lei
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Affiliations: Department of Otolaryngology, The First Affiliated Hospital of Sun Yet-Sen University, Yuexiu, Guangzhou, Guangdong, P.R. China, Department of Otolaryngology, The First People's Hospital of Foshan, Foshan, Guangdong, P.R. China, The Otolaryngological Department, Meizhou People's Hospital, Meizhou, Guangdong, P.R. China, Department of Surgery, The University of Hong Kong, Queen Mary Hospital, Hong Kong, P.R. China, Department of Otolaryngology, The First People's Hospital of Guangzhou, Guangzhou, Guangdong, P.R. China
Published online on: May 16, 2017 https://doi.org/10.3892/or.2017.5645
Pages: 211-220

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Abstract

The present study investigated the expression profile and the function of RP11-169D4.1 and explored its potential mechanisms in laryngeal squamous cell carcinoma. The biological function of RP11-169D4.1 was examined using the MTT assay, flow cytometric analysis, wound healing and transwell assays. The relationship between RP11-169D4.1 and miR-205-5p was discovered by Argonaute 2 protein immunoprecipitation. The target gene of RP11-169D4.1 was CDH1 which was assessed by Pearson's correlation analysis, RT-PCR and western blot assay. We demonstrated that RP11-169D4.1 expression was markedly decreased in LSCC tissues and cell lines. The overexpression of RP11-169D4.1 inhibited the proliferation, migration and invasion of LSCC cell lines as well as promoted apoptosis. We further verified that miR-205-5p had binding sites with RP11‑169D4.1 and that RP11-169D4.1 could regulate the expression of CDH1. Ectopic transfection of RP11-169D4.1 led to a significant reduction in the downstream signaling molecule AKT in LSCC cells. The long non-coding RNA RP11-169D4.1 may serve as a tumor suppressor and a promising therapeutic target in laryngeal cancer, which could inhibit the process of EMT by regulating CDH1.

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