Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway

  • Authors:
    • Xuedong Wang
    • Jianping Zeng
    • Liang Wang
    • Xinjing Zhang
    • Zhiqian Liu
    • Hongyi Zhang
    • Jiahong Dong
  • View Affiliations

  • Published online on: June 6, 2017     https://doi.org/10.3892/or.2017.5699
  • Pages: 141-150
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Abstract

The aim of the present study was carried out to investigate the association of microRNA-133b (miRNA-133b) expression with the prognosis of patients with hepatocellular carcinoma (HCC) after curative hepatectomy. In the present study, the expression of miRNA-133b in HCC tissues was determined to be lower than that noted in the adjacent normal tissues. Overall and disease-free survival of the HCC patients with high miRNA-133b expression was observably extended, compared with the HCC patients with low miRNA‑133b expression. Moreover, the overexpression of miRNA-133b inhibited cell proliferation, increased lactate dehydrogenase (LDH) activity, induced apoptosis and promoted caspase‑3/-8 activities and Bax/Bcl-2 protein expression in HCC cells, whereas the protein expression of epidermal growth factor receptor (EGFR) was significantly decreased. The overexpression of miRNA-133b significantly suppressed PI3K, phosphorylated (p)-Akt and p-mTOR protein expression in HCC cells. GW2974, an EGFR inhibitor, suppressed the protein expression of EGFR, inhibited cell proliferation, increased LDH activity, induced apoptosis and promoted caspase-3/-8 activities and Bax/Bcl-2 protein expression, downregulated PI3K, p-Akt and p-mTOR protein expression in the transfected HCC cells overexpressing miRNA-133b. Taken together, our results indicate that the overexpression of miRNA-133b is associated with the increased survival of HCC patients after curative hepatectomy. The effects of miRNA-133b in HCC are mediated through the EGFR/PI3K/Akt/mTOR signaling pathway.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang X, Zeng J, Wang L, Zhang X, Liu Z, Zhang H and Dong J: Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncol Rep 38: 141-150, 2017.
APA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., & Dong, J. (2017). Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway. Oncology Reports, 38, 141-150. https://doi.org/10.3892/or.2017.5699
MLA
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38.1 (2017): 141-150.
Chicago
Wang, X., Zeng, J., Wang, L., Zhang, X., Liu, Z., Zhang, H., Dong, J."Overexpression of microRNA-133b is associated with the increased survival of patients with hepatocellular carcinoma after curative hepatectomy: Involvement of the EGFR/PI3K/Akt/mTOR signaling pathway". Oncology Reports 38, no. 1 (2017): 141-150. https://doi.org/10.3892/or.2017.5699