Anticancer and apoptosis‑inducing effects of quercetin in vitro and in vivo

Hashemzaei,Mahmoud; Delarami Far,Amin; Yari,Arezoo; Heravi,Reza   Entezari; Tabrizian,Kaveh; Taghdisi,Seyed   Mohammad; Sadegh,Sarvenaz   Ekhtiari; Tsarouhas,Konstantinos; Kouretas,Dimitrios; Tzanakakis,George; Nikitovic,Dragana; Anisimov,Nikita   Yurevich; Spandidos,Demetrios   A.; Tsatsakis,Aristides  M.; Rezaee,Ramin

doi:10.3892/or.2017.5766

Anticancer and apoptosis‑inducing effects of quercetin in vitro and in vivo

Authors:
- Mahmoud Hashemzaei
- Amin Delarami Far
- Arezoo Yari
- Reza Entezari Heravi
- Kaveh Tabrizian
- Seyed Mohammad Taghdisi
- Sarvenaz Ekhtiari Sadegh
- Konstantinos Tsarouhas
- Dimitrios Kouretas
- George Tzanakakis
- Dragana Nikitovic
- Nikita Yurevich Anisimov
- Demetrios A. Spandidos
- Aristides M. Tsatsakis
- Ramin Rezaee
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Affiliations: Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran, Students Research Committee, School of Pharmacy, Zabol University of Medical Sciences, Zabol, Iran, Targeted Drug Delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran, Department of Cardiology, Larissa University Hospital, Larissa, Greece, Department of Biochemistry and Biotechnology, Faculty of Animal Physiology‑Toxicology, University of Thessaly, Larissa, Greece, Department of Anatomy‑Histology‑Embryology, Medical School, University of Crete, Greece, SEC Nanotechnology, Engineering School, Far Eastern Federal University, Vladivostok, Russia, Laboratory of Clinical Virology, Faculty of Medicine, University of Crete, Heraklion, Greece, Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, Heraklion, Greece, Clinical Research Unit, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Published online on: June 28, 2017 https://doi.org/10.3892/or.2017.5766
Pages: 819-828
Copyright: © Hashemzaei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study focused on the elucidation of the putative anticancer potential of quercetin. The anticancer activity of quercetin at 10, 20, 40, 80 and 120 µM was assessed in vitro by MMT assay in 9 tumor cell lines (colon carcinoma CT‑26 cells, prostate adenocarcinoma LNCaP cells, human prostate PC3 cells, pheocromocytoma PC12 cells, estrogen receptor‑positive breast cancer MCF‑7 cells, acute lymphoblastic leukemia MOLT‑4 T‑cells, human myeloma U266B1 cells, human lymphoid Raji cells and ovarian cancer CHO cells). Quercetin was found to induce the apoptosis of all the tested cancer cell lines at the utilized concentrations. Moreover, quercetin significantly induced the apoptosis of the CT‑26, LNCaP, MOLT‑4 and Raji cell lines, as compared to control group (P<0.001), as demonstrated by Annexin V/PI staining. In in vivo experiments, mice bearing MCF‑7 and CT‑26 tumors exhibited a significant reduction in tumor volume in the quercetin‑treated group as compared to the control group (P<0.001). Taken together, quercetin, a naturally occurring compound, exhibits anticancer properties both in vivo and in vitro.

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