miR-125a-5p upregulation suppresses the proliferation and induces the cell apoptosis of lung adenocarcinoma by targeting NEDD9 Retraction in /10.3892/or.2022.8466

Zheng,Hong; Wu,Jianbo; Shi,Jiachen; Lu,Chunya; Wang,Yuanyuan; Sun,Qianqian; Zhang,Guojun; Zhao,Guoqiang

doi:10.3892/or.2017.5812

miR-125a-5p upregulation suppresses the proliferation and induces the cell apoptosis of lung adenocarcinoma by targeting NEDD9

Retraction in: /10.3892/or.2022.8466

Authors:
- Hong Zheng
- Jianbo Wu
- Jiachen Shi
- Chunya Lu
- Yuanyuan Wang
- Qianqian Sun
- Guojun Zhang
- Guoqiang Zhao
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Affiliations: Department of Pathophysiology, Institute of Molecular Medicine, Medical College of Henan University, Kaifeng, Henan 475004, P.R. China, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
Published online on: July 13, 2017 https://doi.org/10.3892/or.2017.5812
Pages: 1790-1796

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Abstract

MicroRNAs (miRNAs) are critical translational regulators that act as oncogenes or tumor-suppressor genes. qRT-PCR assay results showed that the expression levels of miR-125a-5p are lower in lung adenocarcinoma (AD) tissues than expression levels in adjacent non-neoplastic tissues. This relative expression was found to be significantly associated with lymph node metastases. Cell growth, apoptosis, caspase activity and Transwell invasion assay results showed that in two lung adenocarcinoma cell lines transfected with a miR-125a-5p mimic, proliferation and invasion rates were found to be significantly reduced, whereas the apoptosis rate of the miR-125a-5p mimic group was enhanced. Subsequent western blotting and luciferase reporter assays showed that miR-125a-5p is able to bind to putative binding sites within the mRNA 3’ untranslated region (UTR) of neural precursor cell expressed, developmentally downregulated 9 (NEDD9). Our findings suggest that miR-125a-5p may serve as a therapeutic agent for lung adenocarcinoma through its major target, NEDD9.

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