Promotion of glycolysis by HOTAIR through GLUT1 upregulation via mTOR signaling

Wei,Shibo; Fan,Qing; Yang,Liang; Zhang,Xiaodong; Ma,Yingbo; Zong,Zhihong; Hua,Xiangdong; Su,Dongming; Sun,Hongzhi; Li,Hangyu; Liu,Zhen

doi:10.3892/or.2017.5840

Promotion of glycolysis by HOTAIR through GLUT1 upregulation via mTOR signaling

Authors:
- Shibo Wei
- Qing Fan
- Liang Yang
- Xiaodong Zhang
- Yingbo Ma
- Zhihong Zong
- Xiangdong Hua
- Dongming Su
- Hongzhi Sun
- Hangyu Li
- Zhen Liu
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Affiliations: Department of General Surgery, The Shengjing Hospital Affiliated to China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, P.R. China, Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of General Surgery, Liaoning Cancer Hospital and Institute, Shenyang, Liaoning 110042, P.R. China, Center of Metabolic Disease Research, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of General Surgery, The First Hospital Affiliated to Jinzhou Medical College, Jinzhou, Liaoning 121001, P.R. China
Published online on: July 20, 2017 https://doi.org/10.3892/or.2017.5840
Pages: 1902-1908

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Abstract

The long non-coding RNA HOX transcript antisense RNA (HOTAIR) plays a key role in the progression of various carcinomas. However, whether or not HOTAIR influences glucose metabolism and the specific underlying mechanism in hepatocellular carcinoma (HCC) cells remain unclear. In the present study, we found markedly increased HOTAIR expression in 84 HCC tissues and demonstrated that HOTAIR overexpression promoted cell proliferation using Cell Counting Kit-8. The effect on glucose metabolism regulated by HOTAIR in HCC cells was determined by detecting lactate and glucose levels: HOTAIR promoted glycolysis by upregulating glucose transporter isoform 1 (GLUT1) and activating mammalian target of rapamycin (mTOR) signaling, whereas knockdown of HOTAIR suppressed this effect. Our research reveals a novel relationship between HOTAIR and glucose metabolism in HCC cells, and it may be a therapeutic target for diagnosing and treating HCC.

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