B7-H3 is related to tumor progression in ovarian cancer

  • Authors:
    • Jingjing Zhang
    • Lu Liu
    • Sai Han
    • Yi Li
    • Qiuhong Qian
    • Qianqian Zhang
    • Hui Zhang
    • Ziyan Yang
    • Youzhong Zhang
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/or.2017.5858
  • Pages: 2426-2434
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Abstract

B7-H3, a co-stimulatory molecule, has been found expressed in ovarian cancer, but its role and mechanism is not clear. In this study, we further verified the expression of B7-H3 in ovarian carcinoma and normal epithelial ovarian tissues. Three ovarian cancer cell lines, A2780, SKOV3 and HO8910 were selected to explore the effects of B7-H3 on proliferation, apoptosis, migration and invasion. We found that B7-H3 was mainly located in the cytoplasm of ovarian cancer cells as determined by immunofluorescence staining. The ability of cell invasion, migration, proliferation decreased after silencing B7-H3 whereas the apoptosis increased, which was related to the upregulation of Bax, caspase-8, cleaved caspase-8 and the downregulation of Bcl-2, Bcl-xl, matrix metalloproteinase-2 (MMP2) by western blotting. In addition, B7-H3 enhanced the H08910 cell capacities in invasion, migration and proliferation. Expression of the phosphorylation signal transducer and activator of transcription 3 (pStat3) molecules and their upstream molecules phosphorylation Janus kinase 2 (pJak2) were significantly increased. In order to investigate whether B7-H3 plays a role in this pathway, we treated the overexpressed HO8910 cells with AG490 (inhibitors of Jak2). Our findings revealed that B7-H3 affect ovarian cancer progression through the Jak2/Stat3 pathway, indicating that B7-H3 has the potential to be a useful prognostic marker.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Zhang J, Liu L, Han S, Li Y, Qian Q, Zhang Q, Zhang H, Yang Z and Zhang Y: B7-H3 is related to tumor progression in ovarian cancer. Oncol Rep 38: 2426-2434, 2017.
APA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q. ... Zhang, Y. (2017). B7-H3 is related to tumor progression in ovarian cancer. Oncology Reports, 38, 2426-2434. https://doi.org/10.3892/or.2017.5858
MLA
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38.4 (2017): 2426-2434.
Chicago
Zhang, J., Liu, L., Han, S., Li, Y., Qian, Q., Zhang, Q., Zhang, H., Yang, Z., Zhang, Y."B7-H3 is related to tumor progression in ovarian cancer". Oncology Reports 38, no. 4 (2017): 2426-2434. https://doi.org/10.3892/or.2017.5858