EVI5 is a novel independent prognostic predictor in hepatocellular carcinoma after radical hepatectomy

Tang,Jintian; Ou,Jianghua; Xu,Chunyan; Yi,Chao; Xue,Feng; Xu,Lin; Lai,Fenju; Tang,Jianjun; Li,Shengping; Kang,Tiebang; Ding,Wei; Wang,Boqing

doi:10.3892/or.2017.5862

EVI5 is a novel independent prognostic predictor in hepatocellular carcinoma after radical hepatectomy

Authors:
- Jintian Tang
- Jianghua Ou
- Chunyan Xu
- Chao Yi
- Feng Xue
- Lin Xu
- Fenju Lai
- Jianjun Tang
- Shengping Li
- Tiebang Kang
- Wei Ding
- Boqing Wang
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Affiliations: Department of Hepatopancreatobiliary Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, P.R. China, Department of Breast Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, P.R. China, College of Bioscience and Engineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, Jiangxi Agricultural University, Nanchang, Jiangxi, P.R. China, Department of Gastroenterology, Cancer Hospital of Jiangxi Province, Nanchang, Jiangxi, P.R. China, State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, P.R. China
Published online on: August 1, 2017 https://doi.org/10.3892/or.2017.5862
Pages: 2251-2258

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Abstract

The present study explored the correlation of ecotropic viral integration site 5 (EVI5) expression with clinicopathological features and prognosis in hepatocellular carcinoma (HCC). A total of 205 HCC patients were included retrospectively. Quantitative real-time polymerase chain reaction (RT-qPCR) and western blotting were performed to detect the profile of EVI5 expression in HCC cell lines and fresh tissues. Archived paraffin-embedded specimens were investigated for EVI5 expression by immunohistochemistry (IHC). Both the mRNA and protein levels of EVI5 were obviously upregulated in HCC cell lines and tumor tissues. EVI5 protein level was closely associated with the clinicopathological characteristics, including liver function (P=0.013), venous invasion (P=0.015) and TNM stage (P=0.014). Furthermore, univariate analysis showed that the patients with high EVI5 expression indicated shorter overall survival (OS, P<0.001) and recurrence-free survival (RFS, P=0.001) than those with low EVI5 expression. Importantly, high EVI5 expression also exerts predictive power for higher postoperative recurrence rate by stratified analysis. Multivariate Cox regression analysis demonstrated that OS was correlated with both tumor number (P=0.046) and EVI5 expression (P<0.001) and that RFS was correlated with serum AFP (P=0.023), tumor number (P=0.036) and EVI5 expression (P<0.001). Taken together, EVI5 is an useful independent prognostic marker of survival and recurrence in hepatocellular carcinoma.

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