Regulation of UHRF1 by microRNA-378 modulates medulloblastoma cell proliferation and apoptosis

Zhang,Zhen-Yu; Zhu,Bin; Zhao,Xin-Wei; Zhan,Yun-Bo; Bao,Jian-Ji; Zhou,Jin-Qiao; Zhang,Feng-Jiang; Yu,Bin; Liu,Jun; Wang,Yan-Min; Bai,Ya-Hui; Hong,Jin; Liu,Xian-Zhi

doi:10.3892/or.2017.5939

Regulation of UHRF1 by microRNA-378 modulates medulloblastoma cell proliferation and apoptosis

Authors:
- Zhen-Yu Zhang
- Bin Zhu
- Xin-Wei Zhao
- Yun-Bo Zhan
- Jian-Ji Bao
- Jin-Qiao Zhou
- Feng-Jiang Zhang
- Bin Yu
- Jun Liu
- Yan-Min Wang
- Ya-Hui Bai
- Jin Hong
- Xian-Zhi Liu
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Affiliations: Department of Neurosurgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450001, P.R. China, Queen Mary Institute, Nanchang University, Nanchang, Jiangxi 330000, P.R. China, Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
Published online on: September 4, 2017 https://doi.org/10.3892/or.2017.5939
Pages: 3078-3084

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Abstract

A previous study revealed that ubiquitin-like with PHD and RING finger domains 1 (UHRF1) promoted cell proliferation and was a potential biomarker in medulloblastoma (MB). In the present study, we reported that miR-378 inhibited the expression of UHRF1 to affect the proliferation of MB through competitive binding to the same region of its 3'-UTR. We found that the expression of miR-378 was significantly downregulated in MB tissues and inversely correlated with the expression of UHRF1. Western blot analysis revealed that overexpression of miR-378 led to the suppression of UHRF1. Moreover, a dual-luciferase assay demonstrated that miR-378 negatively regulated the activity of target gene UHRF1 by binding to its 3'-UTR. An in vitro assay revealed that overexpression of miR-378 suppressed MB cell proliferation and promoted cell apoptosis. Ectopic expression of UHRF1 rescued miR-378-suppressed cell proliferation and miR-378-promoted cell apoptosis. Collectively, the present study demonstrated that miR-378 could inhibit the proliferation of MB by downregulation of UHRF1 and act as a potential therapeutic target against MB.

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