Open Access

De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells

  • Authors:
    • Magdalena Görtz
    • Uwe Galli
    • Thomas Longerich
    • Margot Zöller
    • Ulrike Erb
    • Peter Schemmer
  • View Affiliations

  • Published online on: September 20, 2017     https://doi.org/10.3892/or.2017.5980
  • Pages: 2697-2704
  • Copyright: © Görtz et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

C4.4A is a glycoprotein that is upregulated in several human malignancies, including colorectal, breast and renal cell carcinomas. Due to its highly restricted expression in healthy tissue, C4.4A was proposed as a potential diagnostic marker. Thus, the present study was designed to evaluate C4.4A expression and function in hepatocellular carcinoma (HCC) for the first time. Immunohistochemistry was performed to detect expression of C4.4A in human sections of healthy liver, primary HCC in the liver and metastatic HCC in the lung. To assess the contribution of C4.4A to HCC progression proliferation, apoptosis, migration and invasion assays were performed with C4.4A knockdown Huh7 and HepG2 cells. C4.4A is absent in healthy liver tissue. However, intense expression was seen in 59% of primary HCCs and strong expression in 80% of HCC lung metastases. C4.4A expression was also observed in human HCC cell lines, which strongly increased under hypoxic conditions. A C4.4A knock-down revealed that C4.4A is involved in both migration and invasion of HCC cells. Taken together, C4.4A expression in both primary and metastatic HCC suggests its potential value as a diagnostic marker for HCC. Due to its absence in healthy liver tissue, C4.4A might even serve as a possible therapeutic target, particularly for metastatic HCC.
View Figures
View References

Related Articles

Journal Cover

November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Görtz M, Galli U, Longerich T, Zöller M, Erb U and Schemmer P: De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells. Oncol Rep 38: 2697-2704, 2017.
APA
Görtz, M., Galli, U., Longerich, T., Zöller, M., Erb, U., & Schemmer, P. (2017). De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells. Oncology Reports, 38, 2697-2704. https://doi.org/10.3892/or.2017.5980
MLA
Görtz, M., Galli, U., Longerich, T., Zöller, M., Erb, U., Schemmer, P."De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells". Oncology Reports 38.5 (2017): 2697-2704.
Chicago
Görtz, M., Galli, U., Longerich, T., Zöller, M., Erb, U., Schemmer, P."De novo synthesis of C4.4A in hepatocellular carcinoma promotes migration and invasion of tumor cells". Oncology Reports 38, no. 5 (2017): 2697-2704. https://doi.org/10.3892/or.2017.5980