MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway

  • Authors:
    • Yanbing Wang
    • Shicai Sun
    • Jianting Xu
    • Yang Luo
    • Ying Xin
    • Xin Zhao
  • View Affiliations

  • Published online on: December 4, 2017     https://doi.org/10.3892/or.2017.6129
  • Pages: 589-596
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Abstract

We explored the function of microRNA-381 on osteosarcoma cell growth and anticancer mechanism for clinic treatment. MicroRNA-381 of osteosarcoma patients was decreased, microRNA-381 levels were more downregulated at stages III/IV than those at stage I/II in osteosarcoma patients. Downregulation of microRNA-381 using anti-microRNA-381 mimics increased cell proliferation, decreased LDH activity and apoptosis, and inhibited caspase‑3/9 activities, Bax/Bcl-2 and p53 protein expression in vitro osteosarcoma cells through upregulation of LRH-1/Wnt/β-catenin signaling pathway. Overexpression of microRNA-381 using microRNA-381 mimics inhibited cell proliferation, induced apoptosis and increased LDH activity, caspase‑3/9 activities, expression of Bax/Bcl-2 and p53 protein in osteosarcoma of in vitro model through downregulation of LRH-1/Wnt/β-catenin signaling pathway. Si‑LRH‑1 promoted the anticancer effects of microRNA-381 on osteosarcoma cell growth through Wnt/β‑catenin signaling pathway. Thus, our data suggested that microRNA-381 suppresses the proliferation of osteosarcoma cells through upregulation of LRH-1/Wnt/β-catenin signaling pathway.
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February-2018
Volume 39 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Wang Y, Sun S, Xu J, Luo Y, Xin Y and Zhao X: MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncol Rep 39: 589-596, 2018.
APA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., & Zhao, X. (2018). MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway. Oncology Reports, 39, 589-596. https://doi.org/10.3892/or.2017.6129
MLA
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39.2 (2018): 589-596.
Chicago
Wang, Y., Sun, S., Xu, J., Luo, Y., Xin, Y., Zhao, X."MicroRNA-381 suppresses the proliferation of osteosarcoma cells through LRH-1/Wnt/β-catenin signaling pathway". Oncology Reports 39, no. 2 (2018): 589-596. https://doi.org/10.3892/or.2017.6129